Accurate quantification of CXCR4-using HIV-1 variants by Illumina deep-sequencing

被引：0

作者：

Raymond, Stephanie ^{[1
,2
]}

Delaugerre, Constance ^{[3
,4
]}

Nicot, Florence ^{[1
]}

Assoumou, Lambert ^{[5
]}

Lancar, Remi ^{[5
]}

Beniguel, Lydie ^{[5
]}

Izopet, Jacques ^{[1
]}

机构：

[1] CPTP, CNRS, UMR 5282, INSERM,U1043, Toulouse, France

[2] CHU Toulouse Purpan, Virol Lab, F-31300 Toulouse, France

[3] Hop St Louis, Assistance Publ Hop Paris, Virol, Paris, France

[4] Univ Paris Diderot, INSERM UMR 941, Sorbonne Paris Cite, Paris, France

[5] Sorbonne Univ, INSERM, IPLESP, Paris, France

来源：

AIDS | 2018年 / 32卷 / 16期

关键词：

CORECEPTOR USAGE; MARAVIROC; TROPISM;

D O I：

10.1097/QAD.0000000000001976

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Next-generation sequencing is a sensitive method for determining HIV-1 tropism but there is a lack of data on the quantification of X4 variants. We evaluated MiSeq and 454 GS-Junior platforms for determining HIV-1 tropism and for quantifying X4 variants. Both platforms were 93% concordant for determining HIV-1 tropism and correlated well for determining the proportion of X4 variants (Spearman correlation, p = 0.748; P < 0.0001). MiSeq Illumina sequencing seems to be well adapted for characterizing X4-containing samples.

引用

页码：2429 / 2431

页数：3

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