Accurate quantification of CXCR4-using HIV-1 variants by Illumina deep-sequencing

被引：0
|
作者：
Raymond, Stephanie [1 ,2 ]
Delaugerre, Constance [3 ,4 ]
Nicot, Florence [1 ]
Assoumou, Lambert [5 ]
Lancar, Remi [5 ]
Beniguel, Lydie [5 ]
Izopet, Jacques [1 ]
机构：
[1] CPTP, CNRS, UMR 5282, INSERM,U1043, Toulouse, France
[2] CHU Toulouse Purpan, Virol Lab, F-31300 Toulouse, France
[3] Hop St Louis, Assistance Publ Hop Paris, Virol, Paris, France
[4] Univ Paris Diderot, INSERM UMR 941, Sorbonne Paris Cite, Paris, France
[5] Sorbonne Univ, INSERM, IPLESP, Paris, France
来源：
AIDS | 2018年 / 32卷 / 16期
关键词：
CORECEPTOR USAGE; MARAVIROC; TROPISM;
D O I：
10.1097/QAD.0000000000001976
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Next-generation sequencing is a sensitive method for determining HIV-1 tropism but there is a lack of data on the quantification of X4 variants. We evaluated MiSeq and 454 GS-Junior platforms for determining HIV-1 tropism and for quantifying X4 variants. Both platforms were 93% concordant for determining HIV-1 tropism and correlated well for determining the proportion of X4 variants (Spearman correlation, p = 0.748; P < 0.0001). MiSeq Illumina sequencing seems to be well adapted for characterizing X4-containing samples.
引用
收藏
页码：2429 / 2431
页数：3
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