Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and ω-transaminase

被引：42

作者：

Seo, Young-Man ^{[1
]}

Mathew, Sam ^{[1
]}

Bea, Han-Seop ^{[1
]}

Khang, Yong-Ho ^{[1
]}

Lee, Sang-Hyeup ^{[2
]}

Kim, Byung-Gee ^{[3
]}

Yun, Hyungdon ^{[1
]}

机构：

[1] Yeungnam Univ, Sch Biotechnol, Gyeongbuk 712749, South Korea

[2] Catholic Univ Daegu, Dept Life Chem, Gyeongbuk 712749, South Korea

[3] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 151742, South Korea

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2012年 / 10卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

KINETIC RESOLUTION; VITREOSCILLA HEMOGLOBIN; ASYMMETRIC-SYNTHESIS; FUSION PROTEIN; DERACEMISATION; DERIVATIVES; HYDROLYSIS; ESTERS;

D O I：

10.1039/c2ob07161d

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A deracemization method was developed to generate optically pure L-homoalanine from racemic homoalanine using D-amino acid oxidase and omega-transaminase. A whole cell reaction using a biphasic system converted 500 mM racemic homoalanine to 485 mM L-homoalanine (>99% ee).

引用

下载

页码：2482 / 2485

页数：4

共 50 条

[31] STRUCTURE OF THE FLAVOENZYME D-AMINO ACID OXIDASE
Bolognesi, M.
Mattevi, M. A.
Vanoni, M. A.
Todone, F.
Curti, B.
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 1996, 52 : C118 - C118
[32] D-amino acid oxidase as an industrial biocatalyst
Pilone, MS
Pollegioni, L
BIOCATALYSIS AND BIOTRANSFORMATION, 2002, 20 (03) : 145 - 159
[33] D-AMINO ACID OXIDASE FROM KIDNEY
BURTON, K
METHODS IN ENZYMOLOGY, 1955, 2 : 199 - 204
[34] INHIBITION OF D-AMINO ACID OXIDASE BY PHENOTHIAZINES
GABAY, S
HARRIS, SR
FEDERATION PROCEEDINGS, 1965, 24 (2P1) : 581 - &
[35] EFFECT OF ETHANOL ON D-AMINO ACID OXIDASE
OHAMA, H
SUGIURA, N
YAGI, K
JOURNAL OF BIOCHEMISTRY, 1973, 73 (05): : 1123 - 1125
[36] REACTION MECHANISM OF D-AMINO ACID OXIDASE
YAGI, K
ADVANCES IN ENZYMOLOGY AND RELATED AREAS OF MOLECULAR BIOLOGY, 1971, 34 : 41 - +
[37] SUBSTRATE COMPETITION FOR D-AMINO ACID OXIDASE
LARUE, TA
BERG, CP
FEDERATION PROCEEDINGS, 1962, 21 (02) : 250 - &
[38] D-Amino acid oxidase: new findings
M. S. Pilone
Cellular and Molecular Life Sciences CMLS, 2000, 57 : 1732 - 1747
[39] Characterization of human D-amino acid oxidase
Molla, G
Sacchi, S
Bernasconi, M
Pilone, MS
Fukui, K
Pollegioni, L
FEBS LETTERS, 2006, 580 (09): : 2358 - 2364
[40] THE SULFHYDRYL CHARACTER OF D-AMINO ACID OXIDASE
FRISELL, WR
HELLERMAN, L
JOURNAL OF BIOLOGICAL CHEMISTRY, 1957, 225 (01) : 53 - 62

← 1 2 3 4 5 →