Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and ω-transaminase

被引：42
|
作者：
Seo, Young-Man [1 ]
Mathew, Sam [1 ]
Bea, Han-Seop [1 ]
Khang, Yong-Ho [1 ]
Lee, Sang-Hyeup [2 ]
Kim, Byung-Gee [3 ]
Yun, Hyungdon [1 ]
机构：
[1] Yeungnam Univ, Sch Biotechnol, Gyeongbuk 712749, South Korea
[2] Catholic Univ Daegu, Dept Life Chem, Gyeongbuk 712749, South Korea
[3] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 151742, South Korea
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2012年 / 10卷 / 12期
基金：
新加坡国家研究基金会;
关键词：
KINETIC RESOLUTION; VITREOSCILLA HEMOGLOBIN; ASYMMETRIC-SYNTHESIS; FUSION PROTEIN; DERACEMISATION; DERIVATIVES; HYDROLYSIS; ESTERS;
D O I：
10.1039/c2ob07161d
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A deracemization method was developed to generate optically pure L-homoalanine from racemic homoalanine using D-amino acid oxidase and omega-transaminase. A whole cell reaction using a biphasic system converted 500 mM racemic homoalanine to 485 mM L-homoalanine (>99% ee).
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页码：2482 / 2485
页数：4
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