Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach

被引：18
|
作者：
Marangi, Marianna [1 ,2 ]
Koehler, Anson V. [2 ]
Zanzani, Sergio A. [3 ]
Manfredi, Maria T. [3 ]
Brianti, Emanuele [4 ]
Giangaspero, Annunziata [1 ]
Gasser, Robin B. [2 ]
机构：
[1] Univ Foggia, Dept Sci Agr Food & Environm, Foggia, Italy
[2] Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic 3010, Australia
[3] Univ Milan, Dept Anim Pathol Hyg & Publ Hlth, Milan, Italy
[4] Univ Messina, Dept Vet Sci, Messina, Italy
来源：
PARASITES & VECTORS | 2015年 / 8卷
基金：
澳大利亚研究理事会; 英国医学研究理事会;
关键词：
Cyclospora; qPCR; Mutation scanning-based sequencing; Non-human primates; Zoonosis; MOLECULAR CHARACTERIZATION; CAYETANENSIS; OUTBREAK; APICOMPLEXA; EIMERIIDAE; INFECTION; FOODBORNE; PATHOGEN; PRIMATES; SPP;
D O I：
10.1186/s13071-015-0872-8
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Background: Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. Methods: A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis. Results: Cyclospora was detected in three Pan troglodytes (13.6%) and nine (9.3%) Macaca fascicularis. Conclusions: The present study represents the first record of Cyclospora in captive primates in Europe, suggesting the presence of Cyclospora cayetanensis, which is transmissible to humans.
引用
收藏
页数：5
相关论文
共 50 条
  • [31] Isolation and PCR-based detection of parvovirus in dogs
    Allmjeed, Dhuha Ismaeel Abdul
    Jumaa, Rawaa Saladdin
    Ibrahim, Osamah Mohammed
    OPEN VETERINARY JOURNAL, 2024, 14 (12) : 3213 - 3218
  • [32] PCR-based detection of Angiostrongylus cantonensis in slugs
    Ovarnstrom, Yvonne
    Bishop, Henry
    Sullivan, James J.
    Hollingsworth, Robert
    da Silva, Alexandre J.
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2005, 73 (06): : 63 - 63
  • [33] PCR-based detection and quantification of mycotoxigenic fungi
    Edwards, SG
    O'Callaghan, J
    Dobson, ADW
    MYCOLOGICAL RESEARCH, 2002, 106 : 1005 - 1025
  • [34] A PCR-based method for tuberculosis detection in wildlife
    Henault, S.
    Karoui, C.
    Boschiroli, M. L.
    NEW DIAGNOSTIC TECHNOLOGY: APPLICATIONS IN ANIMAL HEALTH AND BIOLOGICS CONTROLS, 2006, 126 : 123 - 132
  • [35] Improvement of PCR-Based Detection of ALK Rearrangements
    Mitiushkina, N.
    Tiurin, V.
    Romanko, A.
    Preobrazhenskaya, E.
    Martianov, A.
    Ivantsov, A.
    Yatsuk, O.
    Laidus, T.
    Iyevleva, A.
    Togo, A.
    Imyanitov, E.
    JOURNAL OF THORACIC ONCOLOGY, 2021, 16 (03) : S450 - S451
  • [36] Inverse PCR-based RFLP scanning identifies low-level mutation signatures in colon cells and tumors
    Liu, WH
    Kaur, M
    Wang, G
    Zhu, P
    Zhang, YZ
    Makrigiorgos, GM
    CANCER RESEARCH, 2004, 64 (07) : 2544 - 2551
  • [37] PCR-BASED METHOD TO CREATE A SPECIFIC MUTATION AS A REFERENCE FOR OLIGOMELTING
    GUDNASON, V
    HUMPHRIES, S
    CLINICAL CHEMISTRY, 1991, 37 (12) : 2137 - 2138
  • [38] Development of a quantitative PCR-based method for the detection and monitoring azoxystrobin resistance in Pyricularia oryzae populations
    Kunova, A.
    Pizzatti, C.
    Pasquali, M.
    Cortesi, P.
    PHYTOPATHOLOGY, 2018, 108 (10)
  • [39] Qualitative and Quantitative PCR-Based Detection Methods for Authorized Genetically Modified Cotton Events in India
    Chhabra, Rashmi
    Randhawa, Gurinder Jit
    Bhoge, Rajesh K.
    Singh, Monika
    JOURNAL OF AOAC INTERNATIONAL, 2014, 97 (05) : 1299 - 1309
  • [40] Quantitative PCR-Based Detection Of Typical And Atypical Bacterial Strains In COPD Patients And Relationship To Culture
    Garcha, D. S.
    Thurston, S. J.
    Patel, A. R. C.
    Goldring, J. J.
    McHugh, T. D.
    Donaldson, G. C.
    Wedzicha, J. A.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2011, 183
12345