Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach

被引：18
|
作者：
Marangi, Marianna [1 ,2 ]
Koehler, Anson V. [2 ]
Zanzani, Sergio A. [3 ]
Manfredi, Maria T. [3 ]
Brianti, Emanuele [4 ]
Giangaspero, Annunziata [1 ]
Gasser, Robin B. [2 ]
机构：
[1] Univ Foggia, Dept Sci Agr Food & Environm, Foggia, Italy
[2] Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic 3010, Australia
[3] Univ Milan, Dept Anim Pathol Hyg & Publ Hlth, Milan, Italy
[4] Univ Messina, Dept Vet Sci, Messina, Italy
来源：
PARASITES & VECTORS | 2015年 / 8卷
基金：
澳大利亚研究理事会; 英国医学研究理事会;
关键词：
Cyclospora; qPCR; Mutation scanning-based sequencing; Non-human primates; Zoonosis; MOLECULAR CHARACTERIZATION; CAYETANENSIS; OUTBREAK; APICOMPLEXA; EIMERIIDAE; INFECTION; FOODBORNE; PATHOGEN; PRIMATES; SPP;
D O I：
10.1186/s13071-015-0872-8
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Background: Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. Methods: A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis. Results: Cyclospora was detected in three Pan troglodytes (13.6%) and nine (9.3%) Macaca fascicularis. Conclusions: The present study represents the first record of Cyclospora in captive primates in Europe, suggesting the presence of Cyclospora cayetanensis, which is transmissible to humans.
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页数：5
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