Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach

被引：18

作者：

Marangi, Marianna ^{[1
,2
]}

Koehler, Anson V. ^{[2
]}

Zanzani, Sergio A. ^{[3
]}

Manfredi, Maria T. ^{[3
]}

Brianti, Emanuele ^{[4
]}

Giangaspero, Annunziata ^{[1
]}

Gasser, Robin B. ^{[2
]}

机构：

[1] Univ Foggia, Dept Sci Agr Food & Environm, Foggia, Italy

[2] Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic 3010, Australia

[3] Univ Milan, Dept Anim Pathol Hyg & Publ Hlth, Milan, Italy

[4] Univ Messina, Dept Vet Sci, Messina, Italy

来源：

PARASITES & VECTORS | 2015年 / 8卷

基金：

澳大利亚研究理事会; 英国医学研究理事会;

关键词：

Cyclospora; qPCR; Mutation scanning-based sequencing; Non-human primates; Zoonosis; MOLECULAR CHARACTERIZATION; CAYETANENSIS; OUTBREAK; APICOMPLEXA; EIMERIIDAE; INFECTION; FOODBORNE; PATHOGEN; PRIMATES; SPP;

D O I：

10.1186/s13071-015-0872-8

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Background: Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. Methods: A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis. Results: Cyclospora was detected in three Pan troglodytes (13.6%) and nine (9.3%) Macaca fascicularis. Conclusions: The present study represents the first record of Cyclospora in captive primates in Europe, suggesting the presence of Cyclospora cayetanensis, which is transmissible to humans.

引用

页数：5

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