Transient measurement with an enzyme-immobilized electrode undergoing protein adsorption

被引：3

作者：

Aoyagi, S

Suzuki, Y

Sakai, K

Miyasaka, T

Yoshimi, Y

机构：

[1] Waseda Univ, Dept Chem Engn, Shinjuku Ku, Tokyo 1698555, Japan

[2] Kawasaki Med Sch, Dept Physiol, Kurashiki, Okayama 7010192, Japan

[3] Shibaura Inst Technol, Dept Ind Chem, Minato Ku, Tokyo 1088548, Japan

来源：

ELECTROCHEMISTRY | 2001年 / 69卷 / 04期

关键词：

transient state; glucose; glucose oxidase; on-off control;

D O I：

10.5796/electrochemistry.69.251

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A measurement without the influence of protein adsorption on the sensor surface has been developed using transient current of an enzyme-immobilized electrode. Response current decreases due to protein adsorption on the sensor surface and the enzyme-immobilized membrane causing glucose concentration gradient. By measuring glucose concentration in the transient state while controlling GOD activity, it is possible to prevent the influence of protein adsorption. A needle-type electrode glucose sensor on which enzyme is immobilized with a polyurethane membrane, with electrochemical on-off control of enzyme activity, is capable of accurate measurement of glucose concentration in bovine serum at least for 24 hrs.

引用

页码：251 / 253

页数：3

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