Lipoxygenase-1 mass-transfer coefficient in aqueous two-phase system using spray extraction column

被引：3
|
作者：
Arsalani, V
Rostami, K
Kheirolomoom, A
机构：
[1] Azad Univ, Dept Sci & Res, Tehran 14515755, Iran
[2] Iranian Res Org Sci & Technol, Ctr Biotechnol, Tehran 15819, Iran
[3] Sharif Univ Technol, Dept Petr & Chem Engn, Tehran, Iran
来源：
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH | 2005年 / 44卷 / 19期
关键词：
D O I：
10.1021/ie058001t
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
Extraction of lipoxygenase-1 was investigated by using the aqueous two-phase system formed by sodium sulfate-poly(ethylene glycol)-buffer. The extraction was performed in a 47-mm inner diameter spray column, operating in a semibatch manner. The effects of dispersed-phase now rate and phase compositions on fractional dispersed-phase hold up (epsilon(D)) and volumetric mass-transfer coefficient (K(D)a) were respectively studied. It was found that the hold-up and volumetric mass-transfer coefficient increased with an increase in dispersed-phase velocity and decreased with increasing phase composition. Correlations have been developed by nonlinear regression for estimation of fractional dispersed-phase hold up and volumetric mass-transfer coefficients.
引用
收藏
页码：7469 / 7473
页数：5
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