Estimation of vitamin B12 in plasma by High Performance Liquid Chromatography

被引：3
|
作者：
Hasnat, Fahmida [1 ]
Bhuiyan, Habiba Akhter [1 ]
Misbahuddin, Mir [1 ]
机构：
[1] Bangabandhu Sheikh Mujib Med Univ, Dept Pharmacol, Dhaka, Bangladesh
来源：
BANGLADESH JOURNAL OF PHARMACOLOGY | 2017年 / 12卷 / 03期
关键词：
WATER-SOLUBLE VITAMINS; SEPARATION;
D O I：
10.3329/bjp.v12i3.33108
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
High performance liquid chromatography was used to develop and validate the detection of vitamin B-12 in blood plasma sample. The mobile phase consists of a mixture of 0.025% trifluroacetic acid in deionized water and 30% methanol. The mixture used was adjusted to pH 2.9 and the flow rate was adjusted to 0.5 mL/min. The separation was achieved using C18 column maintained at 30 degrees C temperature and detection of vitamin B-12 was conducted at maximum wavelength 230 nm.
引用
收藏
页码：251 / 255
页数：5
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