Estimation of vitamin B12 in plasma by High Performance Liquid Chromatography

被引：3

作者：

Hasnat, Fahmida ^{[1
]}

Bhuiyan, Habiba Akhter ^{[1
]}

Misbahuddin, Mir ^{[1
]}

机构：

[1] Bangabandhu Sheikh Mujib Med Univ, Dept Pharmacol, Dhaka, Bangladesh

来源：

BANGLADESH JOURNAL OF PHARMACOLOGY | 2017年 / 12卷 / 03期

关键词：

WATER-SOLUBLE VITAMINS; SEPARATION;

D O I：

10.3329/bjp.v12i3.33108

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

High performance liquid chromatography was used to develop and validate the detection of vitamin B-12 in blood plasma sample. The mobile phase consists of a mixture of 0.025% trifluroacetic acid in deionized water and 30% methanol. The mixture used was adjusted to pH 2.9 and the flow rate was adjusted to 0.5 mL/min. The separation was achieved using C18 column maintained at 30 degrees C temperature and detection of vitamin B-12 was conducted at maximum wavelength 230 nm.

引用

页码：251 / 255

页数：5

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