Role of amino acid residues surrounding the phosphorylation site in peptide substrates of G protein-coupled receptor kinase 2 (GRK2)

被引：4

作者：

Asai, Daisuke ^{[1
]}

Murata, Masaharu ^{[2
]}

Toita, Riki ^{[3
]}

Kawano, Takahito ^{[2
]}

Nakashima, Hideki ^{[1
]}

Kang, Jeong-Hun ^{[4
]}

机构：

[1] St Marianna Univ, Sch Med, Dept Microbiol, Sugao 2-16-1 Miyamae, Kawasaki, Kanagawa 2168511, Japan

[2] Kyushu Univ, Fac Med Sci, Dept Adv Med Initiat, Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan

[3] Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, 1-8-31 Midorioka, Ikeda, Osaka 5638577, Japan

[4] Natl Cerebral & Cardiovasc Ctr, Res Inst, Div Biopharmaceut & Pharmacokinet, 5-7-1 Fujishiro Dai, Suita, Osaka 5658565, Japan

来源：

AMINO ACIDS | 2016年 / 48卷 / 12期

关键词：

G protein-coupled receptor kinase; Amino acid residue; Phosphorylation; Cellular signal transduction pathway; Consensus sequence; BETA; IDENTIFICATION; TRAFFICKING; ANTAGONIST; UNVEILS; TUBULIN;

D O I：

10.1007/s00726-016-2345-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of amino acid substitutions was made in a previously identified beta-tubulin-derived GRK2 substrate peptide ((404)DEMEFTEAESNMN(416)) to examine the role of amino acid residues surrounding the phosphorylation site. Anionic amino acid residues surrounding the phosphorylation site played an important role in the affinity for GRK2. Compared to the original peptide, a modified peptide (Ac-EEMEFSEAEANMN-NH2) exhibited markedly higher affinity for GRK2, but very low affinity for GRK5, suggesting that it can be a sensitive and selective peptide for GRK2.

引用

页码：2875 / 2880

页数：6

共 50 条

[11] Clathrin required for phosphorylation and internalization of β2-adrenergic receptor by G protein-coupled receptor kinase 2 (GRK2)
Mangmool, Supachoke
Haga, Tatsuya
Kobayashi, Hiroyuki
Kim, Kyeong-Man
Nakata, Hiroyasu
Nishida, Motohiro
Kurose, Hitoshi
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (42) : 31940 - 31949
[12] G protein-coupled receptor kinase 2 (GRK2) as a multifunctional signaling hub
Petronila Penela
Catalina Ribas
Francisco Sánchez-Madrid
Federico Mayor
Cellular and Molecular Life Sciences, 2019, 76 : 4423 - 4446
[13] Role of the GRK2 extreme amino terminus and active site tether in forming G protein-coupled receptor docking site
Sterne-Marr, Rachel
Michalski, Kevin
Beautrait, Alexandre
Lopez, Thomas
Mannix, Katelynn
McDonald, Devin
Cutter, Amber
Francis, Charnelle
Medina, Christopher
Bouvier, Michel
Tesmer, John
FASEB JOURNAL, 2013, 27
[14] G Protein-Coupled Receptor Kinase 2 (GRK2) and 5 (GRK5) Exhibit Selective Phosphorylation of the Neurotensin Receptor in Vitro
Inagaki, Sayaka
Ghirlando, Rodolfo
Vishnivetskiy, Sergey A.
Homan, Kristoff T.
White, Jim F.
Tesmer, John J. G.
Gurevich, Vsevolod V.
Grisshammer, Reinhard
BIOCHEMISTRY, 2015, 54 (28) : 4320 - 4329
[15] Agonist-dependent phosphorylation of the G protein-coupled receptor kinase 2 (GRK2) by Src tyrosine kfinase
Depto. de Biología Molecular, Ctro. Biol. Molec. Severo Ochoa, Universidad Autónoma, E-28049 Madrid, Spain
J Biol Chem, 48 (34411-34416):
[16] Inhibition of WNT Signaling by G Protein-Coupled Receptor (GPCR) Kinase 2 (GRK2)
Wang, Liming
Gesty-Palmer, Diane
Fields, Timothy A.
Spurney, Robert F.
MOLECULAR ENDOCRINOLOGY, 2009, 23 (09) : 1455 - 1465
[17] New roles of G protein-coupled receptor kinase 2 (GRK2) in cell migration
Penela, Petronila
Ribas, Catalina
Aymerich, Ivette
Mayor, Federico, Jr.
CELL ADHESION & MIGRATION, 2009, 3 (01) : 19 - 23
[18] G protein-coupled receptor kinase 2 (GRK2):: mechanisms of regulation and physiological functions
Aragay, AM
Ruiz-Gómez, A
Penela, P
Sarnago, S
Elorza, A
Jiménez-Sainz, MC
Mayor, F
FEBS LETTERS, 1998, 430 (1-2) : 37 - 40
[19] G protein-coupled receptor kinase 2 (GRK2) modulation and cell cycle progression
Penela, Petronila
Rivas, Veronica
Salcedo, Alicia
Mayor, Federico, Jr.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (03) : 1118 - 1123
[20] Role of G protein-coupled receptor kinase, GRK2, in brown adipose tissue function and differentiation
Vila-Bedmar, R.
Garcia-Guerra, L.
Nieto-Vazquez, I
Mayor, F., Jr.
Lorenzo, M.
Murga, C.
Fernandez-Veledo, S.
DIABETES OBESITY & METABOLISM, 2010, 12 : 35 - 35

← 1 2 3 4 5 →