Aspergillus niger DLFCC-90 Rhamnoside Hydrolase, a New Type of Flavonoid Glycoside Hydrolase

被引：21

作者：

Liu, Tingqiang ^{[1
,2
]}

Yu, Hongshan ^{[1
]}

Zhang, Chunzhi ^{[1
]}

Lu, Mingchun ^{[1
]}

Piao, Yongzhe ^{[3
]}

Ohba, Masashi ^{[4
]}

Tang, Minqian ^{[6
]}

Yuan, Xiaodong ^{[6
]}

Wei, Shenghua ^{[1
]}

Wang, Kan ^{[1
]}

Ma, Anzhou ^{[1
]}

Feng, Xue ^{[1
]}

Qin, Siqing ^{[1
]}

Mukai, Chisato ^{[5
]}

Tsuji, Akira ^{[5
]}

Jin, Fengxie ^{[1
]}

机构：

[1] Dalian Polytech Univ, Coll Biotechnol, Dalian, Peoples R China

[2] Yanbian Univ, Coll Sci, Yanji, Peoples R China

[3] Dalian Nationalities Univ, Coll Life Sci, Dalian, Peoples R China

[4] Kanazawa Univ, Ctr Instrumental Anal, Kanazawa, Ishikawa, Japan

[5] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 920, Japan

[6] TaKaRa Biotechnol Dalian Co Ltd, Dalian, Peoples R China

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 13期

基金：

美国国家科学基金会;

关键词：

ALPHA-L-RHAMNOSIDASE; LACTOBACILLUS-PLANTARUM; PURIFICATION; NARINGINASE; JUICE; CLASSIFICATION;

D O I：

10.1128/AEM.00054-12

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel rutin-alpha-L-rhamnosidase hydrolyzing a-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the alpha-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13.

引用

页码：4752 / 4754

页数：3

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