Aspergillus niger DLFCC-90 Rhamnoside Hydrolase, a New Type of Flavonoid Glycoside Hydrolase

被引:21
|
作者
Liu, Tingqiang [1 ,2 ]
Yu, Hongshan [1 ]
Zhang, Chunzhi [1 ]
Lu, Mingchun [1 ]
Piao, Yongzhe [3 ]
Ohba, Masashi [4 ]
Tang, Minqian [6 ]
Yuan, Xiaodong [6 ]
Wei, Shenghua [1 ]
Wang, Kan [1 ]
Ma, Anzhou [1 ]
Feng, Xue [1 ]
Qin, Siqing [1 ]
Mukai, Chisato [5 ]
Tsuji, Akira [5 ]
Jin, Fengxie [1 ]
机构
[1] Dalian Polytech Univ, Coll Biotechnol, Dalian, Peoples R China
[2] Yanbian Univ, Coll Sci, Yanji, Peoples R China
[3] Dalian Nationalities Univ, Coll Life Sci, Dalian, Peoples R China
[4] Kanazawa Univ, Ctr Instrumental Anal, Kanazawa, Ishikawa, Japan
[5] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 920, Japan
[6] TaKaRa Biotechnol Dalian Co Ltd, Dalian, Peoples R China
基金
美国国家科学基金会;
关键词
ALPHA-L-RHAMNOSIDASE; LACTOBACILLUS-PLANTARUM; PURIFICATION; NARINGINASE; JUICE; CLASSIFICATION;
D O I
10.1128/AEM.00054-12
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A novel rutin-alpha-L-rhamnosidase hydrolyzing a-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the alpha-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13.
引用
收藏
页码:4752 / 4754
页数:3
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