Detection of active Escherichia coli after irradiation by pulsed UV light using a Qβ phage

被引：0
|
作者：
Myriam, Ben Said [1 ]
Otaki, Masahiro [2 ]
Shinobu, Kasama [2 ]
Abdennaceur, Hassen [1 ]
机构：
[1] Water Res & Technol Ctr CERTE, Water Treatment & Recycling Lab LTRE, Tunis 8020, Tunisia
[2] Ochanomizu Univ, Dept Human & Environm Sci, Bunkyo Ku, Tokyo 1128610, Japan
来源：
AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2010年 / 4卷 / 11期
关键词：
Ultra-violet inactivation; viable but non-culturable bacteria; Q beta phage; active bacteria; GROWTH-PHASE; REPLICATION; MECHANISMS; SURVIVAL; BACTERIA;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The infectivity rates of Q beta phage is used as a bio-indicator of the physiological state of host cells and for the detection of active Escherichia coli irradiated by an increasing number of pulsed ultra-violet (UV) light. Indeed, the combination of a conventional method used to measure colony-forming ability of tested bacteria after exposure to an increase number of pulsed UV light, and the simulation of phage adsorption kinetic, can reveal the existence of active bacteria which lose the cultivability in usual culture media, but keep viability and phage susceptibility.
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页码：1128 / 1134
页数：7
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