Highly Selective Fluorescent Probe for Vicinal-Dithiol-Containing Proteins and In Situ Imaging in Living Cells

被引：71

作者：

Huang, Chusen ^{[1
]}

Yin, Qin ^{[1
]}

Zhu, Weiping ^{[1
]}

Yang, Yi ^{[1
]}

Wang, Xin ^{[1
]}

Qian, Xuhong ^{[1
]}

Xu, Yufang ^{[1
]}

机构：

[1] E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Shanghai, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 33期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

fluorescent probes; in situ imaging; protein labeling; redox proteomics; vicinal dithiols; MAMMALIAN-CELLS; FREE-RADICALS; THIOL-GROUPS; ACID; IDENTIFICATION; OXIDATION; STRESS; QUANTIFICATION; DISULFIDES; FLUOROGENS;

D O I：

10.1002/anie.201101317

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：7551 / 7556

页数：6

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