MRI measurement of alanine uptake in a mouse xenograft model of U-87 MG glioblastoma

被引：1
|
作者：
Yang, Seung-Hyun [1 ]
Choi, Yuna [1 ]
Park, Mirae [1 ]
Son, Hye-Young [1 ,2 ]
Huh, Yong-Min [1 ,2 ,3 ,6 ]
Joo, Chan Gyu [4 ,5 ]
机构：
[1] Yonsei Univ, Coll Med, Dept Radiol, Seoul 03722, South Korea
[2] YUHS KRIBB Med Convergence Res Inst, Seoul 03722, South Korea
[3] Yonsei Univ, Coll Med, Dept Biochem & Mol Biol, Seoul 03722, South Korea
[4] Yonsei Univ, Severance Biomed Sci Inst, Coll Med, Seoul 03722, South Korea
[5] Yonsei Univ, Severance Biomed Sci Inst, Coll Med, 50-1 Yonsei Ro, Seoul 03722, South Korea
[6] Yonsei Univ, Coll Med, Dept Radiol, 50-1 Yonsei Ro, Seoul 03722, South Korea
来源：
MAGNETIC RESONANCE IMAGING | 2022年 / 93卷
基金：
新加坡国家研究基金会;
关键词：
MRI; Alanine; cancer metabolism; ASCT2; Amino acid contrast agent; Amino acid transporter; GLUTAMINE; CANCER;
D O I：
10.1016/j.mri.2022.08.015
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
The potential use of alanine as an MRI contrast agent was investigated. The relaxation properties of alanine solutions were measured at 9.4 T. The T-2 relaxivity caused by the chemical exchange (R-2ex) between amine protons and water protons was 0.10 mM(-1) s(2) at 37 ?. As a demonstration, alanine uptake in a mouse xenograft model of U-87 MG glioblastoma was measured using MRI, and was compared with immunohistochemistry staining of ASCT2, a transporter that imports amino acids into cancer cells. Statistically significant (p = 0.0079) differences in ASCT2 distribution were found between regions that show strong and weak alanine uptake in MRI. To better understand the influence of perfusion, the effect of ASCT2 inhibition on the alanine uptake in MRI was investigated, and dynamic contrast enhanced MRI was compared with alanine MRI.
引用
收藏
页码：189 / 194
页数：6
相关论文
共 50 条
  • [31] Antineoplastic effects of deoxypodophyllotoxin, a potent cytotoxic agent of plant origin, on glioblastoma U-87 MG and SF126 cells
    Guerram, Mounia
    Jiang, Zhen-Zhou
    Sun, Lixin
    Zhu, Xiong
    Zhang, Lu-Yong
    PHARMACOLOGICAL REPORTS, 2015, 67 (02) : 245 - 252
  • [32] Multiple cytotoxic effects of gamabufotalin against human glioblastoma cell line U-87
    Yuan, Bo
    Shimada, Ryota
    Xu, Kang
    Han, Lingyu
    Si, Nan
    Zhao, Haiyu
    Bian, Baolin
    Hayashi, Hideki
    Okazaki, Mari
    Takagi, Norio
    CHEMICO-BIOLOGICAL INTERACTIONS, 2019, 314
  • [33] The synthetic peptide LyeTxI-b derived from Lycosa erythrognatha spider venom is cytotoxic to U-87 MG glioblastoma cells
    Mostafa A. L. Abdel-Salam
    Juliana Carvalho-Tavares
    Kamila Sousa Gomes
    Andrea Teixeira-Carvalho
    Gregory T. Kitten
    Johanna Nyffeler
    Felipe F. Dias
    Pablo V. Mendes dos Reis
    Adriano M. C. Pimenta
    Marcel Leist
    Maria Elena de Lima
    Elaine Maria de Souza-Fagundes
    Amino Acids, 2019, 51 : 433 - 449
  • [34] Lopinavir/Ritonavir Treatment Induces Oxidative Stress and Caspase-independent Apoptosis in Human Glioblastoma U-87 MG Cell Line
    Gratton, Rossella
    Tricarico, Paola Maura
    Guimaraes, Rafael Lima
    Celsi, Fulvio
    Crovella, Sergio
    CURRENT HIV RESEARCH, 2018, 16 (02) : 106 - 112
  • [35] Perfusion MRI of U87 brain tumors in a mouse model
    Sun, YP
    Schmidt, NO
    Schmidt, K
    Doshi, S
    Rubin, JB
    Mulkern, RV
    Carroll, R
    Ziu, M
    Erkmen, K
    Poussaint, TY
    Black, P
    Albert, M
    Burstein, D
    Kieran, MW
    MAGNETIC RESONANCE IN MEDICINE, 2004, 51 (05) : 893 - 899
  • [36] The Lichen compounds evernic acid and usnic acid synergize with Temozolomide in the glioblastoma cellline U-87
    Shcherbakova, A.
    Nyugen, L.
    Ulrich-Merzenich, G. S.
    ONCOLOGY RESEARCH AND TREATMENT, 2019, 42 : 275 - 276
  • [37] The enzyme L-isoaspartyl (D-aspartyl) methyltransferase promotes migration and invasion in human U-87 MG and U-251 MG glioblastoma cell lines
    Belkourchia, Fatima
    Desrosiers, Richard R.
    BIOMEDICINE & PHARMACOTHERAPY, 2021, 140
  • [38] High level of low-density lipoprotein receptors enhance hypericin uptake by U-87 MG cells in the presence of LDL
    Kascakova, Slavka
    Nadova, Zuzana
    Mateasik, Anton
    Mikes, Jaromir
    Huntosova, Veronika
    Refregiers, Matthieu
    Sureau, Franck
    Maurizot, Jean-Claude
    Miskovsky, Pavol
    Jancura, Daniel
    PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2008, 84 (01) : 120 - 127
  • [39] Suppression of the proliferation of human U-87 MG glioblastoma cells by new antagonists of growth hormone-releasing hormone in vivo and in vitro
    Jaszberenyi, Miklos
    Schally, Andrew V.
    Block, Norman L.
    Zarandi, Marta
    Cai, Ren-Zhi
    Vidaurre, Irving
    Szalontay, Luca
    Jayakumar, Arumugam R.
    Rick, Ferenc G.
    TARGETED ONCOLOGY, 2013, 8 (04) : 281 - 290
  • [40] Suppression of the proliferation of human U-87 MG glioblastoma cells by new antagonists of growth hormone-releasing hormone in vivo and in vitro
    Miklos Jaszberenyi
    Andrew V. Schally
    Norman L. Block
    Marta Zarandi
    Ren-Zhi Cai
    Irving Vidaurre
    Luca Szalontay
    Arumugam R. Jayakumar
    Ferenc G. Rick
    Targeted Oncology, 2013, 8 : 281 - 290
12345