MRI measurement of alanine uptake in a mouse xenograft model of U-87 MG glioblastoma

被引：1
|
作者：
Yang, Seung-Hyun [1 ]
Choi, Yuna [1 ]
Park, Mirae [1 ]
Son, Hye-Young [1 ,2 ]
Huh, Yong-Min [1 ,2 ,3 ,6 ]
Joo, Chan Gyu [4 ,5 ]
机构：
[1] Yonsei Univ, Coll Med, Dept Radiol, Seoul 03722, South Korea
[2] YUHS KRIBB Med Convergence Res Inst, Seoul 03722, South Korea
[3] Yonsei Univ, Coll Med, Dept Biochem & Mol Biol, Seoul 03722, South Korea
[4] Yonsei Univ, Severance Biomed Sci Inst, Coll Med, Seoul 03722, South Korea
[5] Yonsei Univ, Severance Biomed Sci Inst, Coll Med, 50-1 Yonsei Ro, Seoul 03722, South Korea
[6] Yonsei Univ, Coll Med, Dept Radiol, 50-1 Yonsei Ro, Seoul 03722, South Korea
来源：
MAGNETIC RESONANCE IMAGING | 2022年 / 93卷
基金：
新加坡国家研究基金会;
关键词：
MRI; Alanine; cancer metabolism; ASCT2; Amino acid contrast agent; Amino acid transporter; GLUTAMINE; CANCER;
D O I：
10.1016/j.mri.2022.08.015
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
The potential use of alanine as an MRI contrast agent was investigated. The relaxation properties of alanine solutions were measured at 9.4 T. The T-2 relaxivity caused by the chemical exchange (R-2ex) between amine protons and water protons was 0.10 mM(-1) s(2) at 37 ?. As a demonstration, alanine uptake in a mouse xenograft model of U-87 MG glioblastoma was measured using MRI, and was compared with immunohistochemistry staining of ASCT2, a transporter that imports amino acids into cancer cells. Statistically significant (p = 0.0079) differences in ASCT2 distribution were found between regions that show strong and weak alanine uptake in MRI. To better understand the influence of perfusion, the effect of ASCT2 inhibition on the alanine uptake in MRI was investigated, and dynamic contrast enhanced MRI was compared with alanine MRI.
引用
收藏
页码：189 / 194
页数：6
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