shRNA against PTEN promotes neurite outgrowth of cortical neurons and functional recovery in spinal cord contusion rats

被引：1
|
作者：
Zhou, Hengxing [1 ]
Li, Xueying [2 ]
Wu, Qiang [3 ]
Li, Fuyuan [1 ]
Fu, Zheng [2 ]
Liu, Chang [4 ]
Liang, Zhipin [4 ]
Chu, Tianci [1 ]
Wang, Tianyi [1 ]
Lu, Lu [1 ]
Ning, Guangzhi [1 ]
Kong, Xiaohong [4 ]
Feng, Shiqing [1 ]
机构：
[1] Tianjin Med Univ, Gen Hosp, Dept Orthopaed, Tianjin 300052, Peoples R China
[2] Tianjin Med Univ, Key Lab Immuno Microenvironm & Dis, Educ Minist China, Dept Immunol, Tianjin 300070, Peoples R China
[3] Tianjin Univ Tradit Chinese Med, Teaching Hosp 1, Dept Orthopaed, Tianjin 300193, Peoples R China
[4] Nankai Univ, Sch Med, Tianjin 300071, Peoples R China
来源：
REGENERATIVE MEDICINE | 2015年 / 10卷 / 04期
基金：
中国国家自然科学基金;
关键词：
axon growth/regeneration; functional recovery; PTEN; RNA interference; spinal cord injury; CHONDROITIN SULFATE PROTEOGLYCANS; RNA INTERFERENCE; STEM-CELLS; IN-VITRO; AXON REGENERATION; GROWTH CONE; INJURY; EXPRESSION; INHIBITION; SURVIVAL;
D O I：
10.2217/RME.14.88
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Aim: To explore neurite growth/regeneration and spinal cord injury repair after PTEN silencing via lentivirus-mediated RNAi. Materials & Methods: Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of neurites were calculated. Lentivirus was locally injected into spinal cord contusion rats. The functional recovery and immunohistochemical staining were analyzed. Results: Neurites with PTEN silencing exhibited significant enhancements in elongation, initiation and crossing ability when they encountered chondroitin sulfate proteoglycans in vitro. In vivo PTEN silencing improved functional recovery significantly, and promoted axon and synapse formation, but not scar formation. Conclusions: PTEN silencing may be promising for spinal cord injury repair.
引用
收藏
页码：411 / 429
页数：19
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