shRNA against PTEN promotes neurite outgrowth of cortical neurons and functional recovery in spinal cord contusion rats

被引：1
|
作者：
Zhou, Hengxing [1 ]
Li, Xueying [2 ]
Wu, Qiang [3 ]
Li, Fuyuan [1 ]
Fu, Zheng [2 ]
Liu, Chang [4 ]
Liang, Zhipin [4 ]
Chu, Tianci [1 ]
Wang, Tianyi [1 ]
Lu, Lu [1 ]
Ning, Guangzhi [1 ]
Kong, Xiaohong [4 ]
Feng, Shiqing [1 ]
机构：
[1] Tianjin Med Univ, Gen Hosp, Dept Orthopaed, Tianjin 300052, Peoples R China
[2] Tianjin Med Univ, Key Lab Immuno Microenvironm & Dis, Educ Minist China, Dept Immunol, Tianjin 300070, Peoples R China
[3] Tianjin Univ Tradit Chinese Med, Teaching Hosp 1, Dept Orthopaed, Tianjin 300193, Peoples R China
[4] Nankai Univ, Sch Med, Tianjin 300071, Peoples R China
来源：
REGENERATIVE MEDICINE | 2015年 / 10卷 / 04期
基金：
中国国家自然科学基金;
关键词：
axon growth/regeneration; functional recovery; PTEN; RNA interference; spinal cord injury; CHONDROITIN SULFATE PROTEOGLYCANS; RNA INTERFERENCE; STEM-CELLS; IN-VITRO; AXON REGENERATION; GROWTH CONE; INJURY; EXPRESSION; INHIBITION; SURVIVAL;
D O I：
10.2217/RME.14.88
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Aim: To explore neurite growth/regeneration and spinal cord injury repair after PTEN silencing via lentivirus-mediated RNAi. Materials & Methods: Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of neurites were calculated. Lentivirus was locally injected into spinal cord contusion rats. The functional recovery and immunohistochemical staining were analyzed. Results: Neurites with PTEN silencing exhibited significant enhancements in elongation, initiation and crossing ability when they encountered chondroitin sulfate proteoglycans in vitro. In vivo PTEN silencing improved functional recovery significantly, and promoted axon and synapse formation, but not scar formation. Conclusions: PTEN silencing may be promising for spinal cord injury repair.
引用
收藏
页码：411 / 429
页数：19
相关论文
共 50 条
  • [21] Fenretinide Promotes Functional Recovery and Tissue Protection after Spinal Cord Contusion Injury in Mice
    Lopez-Vales, Ruben
    Redensek, Adriana
    Skinner, Thomas A. A.
    Rathore, Khizr I.
    Ghasemlou, Nader
    Wojewodka, Gabriella
    DeSanctis, Juan
    Radzioch, Danuta
    David, Samuel
    JOURNAL OF NEUROSCIENCE, 2010, 30 (09): : 3220 - 3226
  • [22] Neurite outgrowth in developing mouse spinal cord neurons is modulated by glycine receptors
    Tapia, JC
    Cárdenas, AM
    Nualart, F
    Mentis, GZ
    Navarrete, R
    Aguayo, LG
    NEUROREPORT, 2000, 11 (13) : 3007 - 3010
  • [23] MAG and MOG enhance neurite outgrowth of embryonic mouse spinal cord neurons
    Turnley, AM
    Bartlett, PF
    NEUROREPORT, 1998, 9 (09) : 1987 - 1990
  • [24] Ciliary neurotrophic factor stimulates neurite outgrowth from spinal cord neurons
    Oyesiku, NM
    Wigston, DJ
    JOURNAL OF COMPARATIVE NEUROLOGY, 1996, 364 (01) : 68 - 77
  • [25] Phospholipase D promotes neurite outgrowth in spinal and cortical neurons: evidence using inhibitory anti-PLD antibodies
    Vidulescu, CF
    Brailoiu, E
    Tuluc, F
    Gaman, L
    Dun, NJ
    Kunapuli, SP
    Clejan, S
    FASEB JOURNAL, 2005, 19 (04): : A290 - A290
  • [26] A novel role for PTEN in the inhibition of neurite outgrowth by myelin-associated glycoprotein in cortical neurons
    Perdigoto, Ana Luisa
    Chaudhry, Nagarathnamma
    Barnes, Gregory N.
    Filbin, Marie T.
    Carter, Bruce D.
    MOLECULAR AND CELLULAR NEUROSCIENCE, 2011, 46 (01) : 235 - 244
  • [27] Midkine overcomes neurite outgrowth inhibition of chondroitin sulfate proteoglycan without glial activation and promotes functional recovery after spinal cord injury
    Muramoto, Akio
    Imagama, Shiro
    Natori, Takamitsu
    Wakao, Norimitsu
    Ando, Kei
    Tauchi, Ryoji
    Hirano, Kenichi
    Shinjo, Ryuichi
    Matsumoto, Tomohiro
    Ishiguro, Naoki
    Kadomatsu, Kenji
    NEUROSCIENCE LETTERS, 2013, 550 : 150 - 155
  • [28] Nimodipine Promotes Functional Recovery After Spinal Cord Injury in Rats
    Guo, Fangliang
    Zheng, Xiaolong
    He, Ziyu
    Zhang, Ruoying
    Zhang, Song
    Wang, Minghuan
    Chen, Hong
    Wang, Wei
    FRONTIERS IN PHARMACOLOGY, 2021, 12
  • [29] Local Injection of Lentivirus Encoding LINGO-1-shRNA Promotes Functional Recovery in Rats With Complete Spinal Cord Transection
    Cen, Jingsheng
    Wu, Hongfu
    Wang, Jue
    Ren, Xianyue
    Zhang, Hongwu
    Wang, Jingnan
    Wan, Yong
    Deng, Yubin
    SPINE, 2013, 38 (19) : 1632 - 1639
  • [30] DNA vaccine against NgR promotes functional recovery after spinal cord injury in adult rats
    Yu, Panpan
    Huang, Lidong
    Zou, Jian
    Zhu, Huiqing
    Wang, Xiaofei
    Yu, Zhihua
    Xu, Xiao-Ming
    Lu, Pei-Hua
    BRAIN RESEARCH, 2007, 1147 : 66 - 76
12345