The effect of Ni(II) on properties of bulking activated sludge and microbial analysis of sludge using 16S rDNA gene

被引：19
|
作者：
Li, Jia [1 ,2 ]
Liu, Yan [1 ]
Zhang, Tong [2 ]
Wang, Ling [1 ]
Liu, Xiang [1 ]
Dai, Ruihua [1 ]
机构：
[1] Fudan Univ, Dept Environm Sci & Engn, Shanghai 200433, Peoples R China
[2] Univ Hong Kong, Environm Biotechnol Lab, Dept Civil Engn, Hong Kong, Hong Kong, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 04期
基金：
美国国家科学基金会;
关键词：
Nickel(II); Settling property; Dewatering ability; Microbial community; 16S rDNA; NICKEL-RESISTANT BACTERIA; POLYMERIC SUBSTANCES EPS; FLOC-FORMING BACTERIA; PHYLOGENETIC DIVERSITY; METAL TOXICITY; FLOCCULATION; COMMUNITIES; BIOFILM; PERFORMANCE; POPULATION;
D O I：
10.1016/j.biortech.2010.12.022
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
This study investigated the effect of nickel on properties and microbial community of bulking activated sludge when 60-240 mg/L Ni(II) was dosed continuously in a sequencing batch reactor (SBR) over 350 days. Results showed that 120 mg/L nickel did not significantly inhibited removal of organic pollutant by activated sludge. However, the system was completely upset when 240 mg/L Ni(II) was dosed. Improvement of settling and dewatering ability was also observed with the addition of Ni(II). In addition, investigations by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rDNA of bacteria strain demonstrated that Ni(II) significantly affected microbial community of bulking activated sludge, judging from the elimination of original species and emergence of possible new nickel-resistant bacteria. The effect of nickel on shift of microbial community was an important cause resulted in the improvement of sludge properties in this bulking activated sludge system. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：3783 / 3789
页数：7
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