The effect of Ni(II) on properties of bulking activated sludge and microbial analysis of sludge using 16S rDNA gene

被引：19
|
作者：
Li, Jia [1 ,2 ]
Liu, Yan [1 ]
Zhang, Tong [2 ]
Wang, Ling [1 ]
Liu, Xiang [1 ]
Dai, Ruihua [1 ]
机构：
[1] Fudan Univ, Dept Environm Sci & Engn, Shanghai 200433, Peoples R China
[2] Univ Hong Kong, Environm Biotechnol Lab, Dept Civil Engn, Hong Kong, Hong Kong, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 04期
基金：
美国国家科学基金会;
关键词：
Nickel(II); Settling property; Dewatering ability; Microbial community; 16S rDNA; NICKEL-RESISTANT BACTERIA; POLYMERIC SUBSTANCES EPS; FLOC-FORMING BACTERIA; PHYLOGENETIC DIVERSITY; METAL TOXICITY; FLOCCULATION; COMMUNITIES; BIOFILM; PERFORMANCE; POPULATION;
D O I：
10.1016/j.biortech.2010.12.022
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
This study investigated the effect of nickel on properties and microbial community of bulking activated sludge when 60-240 mg/L Ni(II) was dosed continuously in a sequencing batch reactor (SBR) over 350 days. Results showed that 120 mg/L nickel did not significantly inhibited removal of organic pollutant by activated sludge. However, the system was completely upset when 240 mg/L Ni(II) was dosed. Improvement of settling and dewatering ability was also observed with the addition of Ni(II). In addition, investigations by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rDNA of bacteria strain demonstrated that Ni(II) significantly affected microbial community of bulking activated sludge, judging from the elimination of original species and emergence of possible new nickel-resistant bacteria. The effect of nickel on shift of microbial community was an important cause resulted in the improvement of sludge properties in this bulking activated sludge system. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：3783 / 3789
页数：7
相关论文
共 50 条
  • [31] Insights into the effect of nickel (Ni(II)) on the performance, microbial enzymatic activity and extracellular polymeric substances of activated sludge
    Ma, Bingrui
    Li, Zhiwei
    Wang, Sen
    Liu, Zhaozhe
    Li, Shanshan
    She, Zonglian
    Yu, Naling
    Zhao, Changkun
    Jin, Chunji
    Zhao, Yangguo
    Guo, Liang
    Gao, Mengchun
    ENVIRONMENTAL POLLUTION, 2019, 251 : 81 - 89
  • [32] Analysis of bacterial community structure using 16S rDNA analysis
    Moffett, BF
    Walsh, KA
    Harris, JA
    Hill, TCJ
    ANAEROBE, 2000, 6 (02) : 129 - 131
  • [33] Systematic analysis of biochemical performance and the microbial community of an activated sludge process using ozone-treated sludge for sludge reduction
    Yan, Sang-Tian
    Zheng, Hao
    Li, An
    Zhang, Xue
    Xing, Xin-Hui
    Chu, Li-Bing
    Ding, Guoji
    Sun, Xu-Lin
    Jurcik, Benjamin
    BIORESOURCE TECHNOLOGY, 2009, 100 (21) : 5002 - 5009
  • [34] Simple assay for colorimetric quantification of unamplified bacterial 16S rRNA in activated sludge using gold nanoprobes
    Nakajima, Meri
    Hirano, Reiko
    Okabe, Satoshi
    Satoh, Hisashi
    Chemosphere, 2021, 263
  • [35] Simple assay for colorimetric quantification of unamplified bacterial 16S rRNA in activated sludge using gold nanoprobes
    Nakajima, Meri
    Hirano, Reiko
    Okabe, Satoshi
    Satoh, Hisashi
    CHEMOSPHERE, 2021, 263
  • [36] Detection of sphingomonads and in situ identification in activated sludge using 16S rRNA-targeted oligonucleotide probes
    Inst. F. Angewandte Mikrobiologie, Justus-Liebig-Universitat Giessen, Senckenbergstr 3, D-35390 Giessen, Germany
    J. Ind. Microbiol. Biotechnol., 4-5 (261-267):
  • [37] Detection of sphingomonads and in situ identification in activated sludge using 16S rRNA-targeted oligonucleotide probes
    Neef, A
    Witzenberger, R
    Kämpfer, P
    JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 1999, 23 (4-5) : 261 - 267
  • [38] Effect of phosphorus limitation on microbial floc structure and gene expression in activated sludge
    Liu, J. R.
    Liu, C. T.
    Edwards, E. A.
    Liss, S. N.
    WATER SCIENCE AND TECHNOLOGY, 2006, 54 (01) : 247 - 255
  • [39] The microbial community in a 2,4-dinitrophenol-digesting reactor as revealed by 16S rDNA gene analysis
    Kimura, N
    Shinozaki, Y
    Lee, TH
    Yonezawa, Y
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2003, 96 (01) : 70 - 75
  • [40] Metagenomic and Metatranscriptomic Analysis of Microbial Community Structure and Gene Expression of Activated Sludge
    Yu, Ke
    Zhang, Tong
    PLOS ONE, 2012, 7 (05):
12345