A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages

被引：9
|
作者：
Istrate, Oana Maria [1 ]
Rotariu, Lucian [1 ,2 ]
Bala, Camelia [1 ,2 ]
机构：
[1] Univ Bucharest, LaborQ, Bucharest 030018, Romania
[2] Univ Bucharest, Dept Analyt Chem, Bucharest 030018, Romania
来源：
SENSORS | 2021年 / 21卷 / 19期
关键词：
alcohol dehydrogenase; biosensor; screen-printed electrode; ethanol; PRINTED CARBON ELECTRODE; ALCOHOL-DEHYDROGENASE; CONDUCTING POLYMER; MASS-SPECTROMETRY; NADH; IMMOBILIZATION; FERMENTATION; GASOLINE;
D O I：
10.3390/s21196510
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol-gel matrix. Working parameters such as applied potential, pH, NAD(+) concentration, storage conditions were optimized. A response range between 0.05 and 2 mM was found with a sensitivity of 13.45 & PLUSMN; 0.67 mu A/mM & BULL;cm(2) and a detection limit of 20 mu M. The developed biosensor was used to detect ethanol in commercial beverages with good accuracy.
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页数：11
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