Impact of electrospray ion source platforms on matrix effect due to plasma phospholipids in the determination of rivastigmine by LC-MS/MS

Background: This study evaluates the performance of three electrospray ionization source designs to monitor the interference of plasma phospholipids for reliable estimation of rivastigmine by LC-MS/MS for method ruggedness. The variation in the area response due to matrix effects was assessed by post-column infusion, post-extraction spiking and standard-line slope methods. Results: The observed interference due to coeluting phospholipids (m/z: 524.0/184.0) at the retention time of rivastigmine was 39.5, 12.9 and 0.4% using angular spray, orthogonal spray and dual orthogonal ion source spray design, respectively. Similarly, %CV for standard line slopes was 6.9, 4.6 and 2.0, respectively. Conclusion: Z-spray source design provided better and efficient transfer of gas phase ions into the mass analyzer compared with angular and orthogonal spray. The study showed that Z-spray ion source provided minimum interference from phospholipids compared with other ion source designs.