Duration of sample storage dramatically alters expression of the human immunodeficiency virus coreceptors CXCR4 and CCR5

被引：15
|
作者：
Shalekoff, S
Tiemessen, CT
机构：
[1] Univ Witwatersrand, Dept Virol, ZA-2050 Witwatersrand, South Africa
[2] Natl Inst Virol, AIDS Virus Res Unit, Johannesburg, South Africa
来源：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2001年 / 8卷 / 02期
关键词：
D O I：
10.1128/CDLI.8.2.432-436.2001
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Expression of the chemokine receptors CXCR4 and CCR5 was monitored using EDTA-anticoagulated whole blood held for different time periods prior to fluorescent-antibody staining. When left overnight CXCR4 expression on leukocytes was substantially increased, whereas CCR5 expression was reduced. The results were similar when heparin and acid-citrate-dextrose were used as anticoagulants.
引用
收藏
页码：432 / 436
页数：5
相关论文
共 50 条
  • [31] Interclade neutralization and enhancement of human immunodeficiency virus type 1 identified by an assay using HeLa cells expressing both CD4 receptor and CXCR4/CCR5 coreceptors
    Barin, F
    Brunet, S
    Brand, D
    Moog, C
    Peyre, R
    Damond, F
    Charneau, P
    Barre-Sinoussi, F
    JOURNAL OF INFECTIOUS DISEASES, 2004, 189 (02): : 322 - 327
  • [32] Cooperation of multiple CCR5 coreceptors is required for infections by human immunodeficiency virus type 1
    Kuhmann, SE
    Platt, EJ
    Kozak, SL
    Kabat, D
    JOURNAL OF VIROLOGY, 2000, 74 (15) : 7005 - 7015
  • [33] CCR5 and CXCR4 usage by non-clade B human immunodeficiency virus type 1 primary isolates
    Thompson, DAD
    Cormier, EG
    Dragic, T
    JOURNAL OF VIROLOGY, 2002, 76 (06) : 3059 - 3064
  • [34] Differential regulation of CXCR4 and CCR5 endocytosis
    Signoret, N
    Rosenkilde, MM
    Klasse, PJ
    Schwartz, TW
    Malim, MH
    Hoxie, JA
    Marsh, M
    JOURNAL OF CELL SCIENCE, 1998, 111 : 2819 - 2830
  • [35] Cellular expression of functional chemokine receptor CCR5 and CXCR4 in human embryonic neurons
    Boutet, A
    Salim, H
    Leclerc, P
    Tardieu, M
    NEUROSCIENCE LETTERS, 2001, 311 (02) : 105 - 108
  • [36] Upregulation of surface feline CXCR4 expression following ectopic expression of CCR5: Implications for studies of the cell tropism of feline immunodeficiency virus
    Willett, BJ
    Cannon, CA
    Hosie, MJ
    JOURNAL OF VIROLOGY, 2002, 76 (18) : 9242 - 9252
  • [37] Up-Regulation of CCR5 and CXCR4 Expression on Human Monocytes by Interferon Gamma
    陆韵
    刘祖强
    陈应华
    TsinghuaScienceandTechnology, 2003, (04) : 440 - 444
  • [38] Combination of CCR5 and CXCR4 inhibitors in therapy of human immunodeficiency virus type 1 infection: In vitro studies of mixed virus infections
    Rusconi, S
    Catamancio, SL
    Citterio, P
    Bulgheroni, E
    Croce, F
    Herrmann, SH
    Offord, RE
    Galli, M
    Hirsch, MS
    JOURNAL OF VIROLOGY, 2000, 74 (19) : 9328 - 9332
  • [39] Putative cholesterol recognition amino acid consensus (CRAC) motif in HIV coreceptors CXCR4 and CCR5
    Zhukovsky, Mikhail A.
    Ott, Albrecht
    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2011, 40 : 205 - 206
  • [40] Biological significance of the expression of HIV related chemokine coreceptors (CCR5 and CXCR4) and endogenous secretion of chemokines by human hematopoietic cell lines.
    Majka, M
    Rozmyslowicz, T
    Honczarenko, M
    Lee, B
    Ratajczak, J
    Wasik, M
    Gaulton, GN
    Gewirtz, AM
    Silberstein, LE
    Ratajczak, MZ
    BLOOD, 1999, 94 (10) : 618A - 618A
12345