HR-MAS NMR analysis of biological tissues: a critical report

被引：6

作者：

Desmoulin, Franck ^{[1
]}

Bon, Delphine ^{[1
]}

Martino, Robert ^{[1
]}

Malet-Martino, Myriam ^{[1
]}

机构：

[1] Univ Toulouse 3, Grp RMN Biomed, Lab SPCMIB, UMR 5068, F-31062 Toulouse 9, France

来源：

COMPTES RENDUS CHIMIE | 2008年 / 11卷 / 4-5期

关键词：

NMR; HR-MAS; tissue; extract; freezing; quantitation;

D O I：

10.1016/j.crci.2007.09.004

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the last five years, the HR-MAS NMR technique has been widely used to explore biological tissues from animals and plants. Numerous advantages attributed to HR-MAS have motivated its use in the metabolomic field, in particular for human pathological biopsies. However, a practical evaluation of HR-MAS on a wide experimental range (in vivo animals, fresh or freezing tissues) reveals logistic, sanitary, and analytical disadvantages. The main drawback concerns the high difficulty to perform correct metabolite quantitation. Thus, for some studies, the NMR analysis of tissue extract was preferred.

引用

页码：423 / 433

页数：11

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