Influence of codon usage on the immunogenicity of a DNA vaccine against tetanus

被引：47
|
作者：
Stratford, R [1 ]
Douce, G [1 ]
Zhang-Barber, L [1 ]
Fairweather, N [1 ]
Eskola, J [1 ]
Dougan, G [1 ]
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ, England
来源：
VACCINE | 2000年 / 19卷 / 7-8期
基金：
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词：
DNA vaccines; tetanus; codon usage; murine model;
D O I：
10.1016/S0264-410X(00)00246-2
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Two related DNA vaccine vector plasmids, harbouring either wild-type (pcDNA3/ntetC) or synthetic codon optimised (pcDNA3/stetC) DNA encoding fragment C (TetC) of tetanus toxin were constructed. COS-7 cells transformed with pcDNA3/ stetC reproducibly expressed higher levels of TetC than similar cells transformed with pcDNA3/ntetC. BALB/c mice immunised intramuscularly with pcDNA3/stetC produced significantly higher levels of anti-TetC antibodies in their serum in the weeks following vaccination compared to mice immunised with pcDNA3/ntetC, even when differences in the CpG content between the two sequences were controlled for using non-expressing DNA. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：810 / 815
页数：6
相关论文
共 50 条
  • [41] Immunogenicity, reactogenicity and consistency of production of a Brazilian combined vaccine against diphtheria, tetanus, pertussis and Haemophilus influenzae type b
    Martins, Reinaldo de Menezes
    Bastos Camacho, Luiz Antonio
    Marcovistz, Rugimar
    Guimaraes de Noronha, Tatiana
    Sousa Maia, Maria de Lourdes
    dos Santos, Eliane Matos
    Barbosa, Glayse Glayde
    Vieira da Silva, Andrea Marques
    Neves Feliciano de Souza, Patricia Cristina
    Ferreira Lemos, Maria Cristina
    Homma, Akira
    MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2008, 103 (07): : 711 - 718
  • [42] Impact of codon usage modification on T cell immunogenicity and longevity of HIV-1 gag-specific DNA vaccines
    Bojak, A
    Wild, J
    Deml, L
    Wagner, R
    INTERVIROLOGY, 2002, 45 (4-6) : 275 - 286
  • [43] Safety and immunogenicity of single dose of tetanus-diphtheria (Td) vaccine among non/partially immune children against diphtheria and/or tetanus, Hyderabad, India, 2007
    Bitragunta, Sailaja
    Murhekar, Manoj V.
    Chakravarti, Anita
    Verma, Vikas
    Namjoshi, Gajanan S.
    Parekh, Sameer S.
    Sharma, Hitt J.
    Kumar, B. Kishore
    Gupte, Mohan D.
    VACCINE, 2010, 28 (37) : 5934 - 5938
  • [44] IMMUNOGENICITY OF VACCINE AGAINST ENZOOTIC ABORTION OF EWES
    GAJDOSOVA, E
    KOVACOVA, E
    KAZAR, J
    KOLCUNOVA, A
    SABO, A
    VETERINARNI MEDICINA, 1994, 39 (10) : 589 - 596
  • [45] Immunogenicity of a subunit vaccine against Bacillus anthracis
    Chichester, Jessica A.
    Musiychuk, Konstantin
    de la Rosa, Patricia
    Horsey, April
    Stevenson, Natalie
    Ugulava, Natalia
    Rabindran, Shailaja
    Palmer, Gene A.
    Mett, Vadim
    Yusibov, Vidadi
    VACCINE, 2007, 25 (16) : 3111 - 3114
  • [46] Codon Usage and Splicing Jointly Influence mRNA Localization
    Mordstein, Christine
    Savisaar, Rosina
    Young, Robert S.
    Bazile, Jeanne
    Talmane, Lana
    Luft, Juliet
    Liss, Michael
    Taylor, Martin S.
    Hurst, Laurence D.
    Kudla, Grzegorz
    CELL SYSTEMS, 2020, 10 (04) : 351 - +
  • [47] Immunogenicity and protective efficacy of a tuberculosis DNA vaccine
    Huygen, K
    Content, J
    Denis, O
    Montgomery, DL
    Yawman, AM
    Deck, RR
    DeWitt, CM
    Orme, IM
    Baldwin, S
    DSouza, C
    Drowart, A
    Lozes, E
    Vandenbussche, P
    VanVooren, JP
    Liu, MA
    Ulmer, JB
    NATURE MEDICINE, 1996, 2 (08) : 893 - 898
  • [48] Molecular mechanisms for enhanced DNA vaccine immunogenicity
    Li, Lei
    Petrovsky, Nikolai
    EXPERT REVIEW OF VACCINES, 2016, 15 (03) : 313 - 329
  • [49] Reduced immunogenicity of DNA vaccine plasmids in mixtures
    Sedegah, M
    Charoenvit, Y
    Minh, L
    Belmonte, M
    Majam, VF
    Abot, S
    Ganeshan, H
    Kumar, S
    Bacon, DJ
    Stowers, A
    Narum, DL
    Carucci, DJ
    Rogers, WO
    GENE THERAPY, 2004, 11 (05) : 448 - 456
  • [50] Reduced immunogenicity of DNA vaccine plasmids in mixtures
    M Sedegah
    Y Charoenvit
    L Minh
    M Belmonte
    VF Majam
    S Abot
    H Ganeshan
    S Kumar
    DJ Bacon
    A Stowers
    DL Narum
    DJ Carucci
    WO Rogers
    Gene Therapy, 2004, 11 : 448 - 456
12345