Cytotoxic activity against prelabelled RTG-2 cells in the turbot, Scophthalmus maximus (L.)

被引:0
|
作者
Tafalla, C [1 ]
Figueras, A [1 ]
Novoa, B [1 ]
机构
[1] CSIC, Inst Invest Marinas, Vigo 36208, Spain
关键词
turbot; leucocytes; cytotoxicity; fish; non-specific natural cytotoxic cells; immune system;
D O I
暂无
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Leucocytes derived from head kidney, blood and spleen of turbot, Scophthalmus maximus (L.), exhibited cytotoxic activities against an established cell line (RTG-2) derived from rainbow trout, Oncorhynchus mykiss (Walbaum). The optimal effector:target ratio, temperature and incubation period for the activity was determined. Cytotoxicity was not caused by released factors, as effector:target contact was needed. Visualization of the cultures under the transmission electron microscope corroborated the contact between leucocytes and target cells and suggested the implication of monocyte-like cells in this cell-mediated cytotoxicity.
引用
收藏
页码:169 / 175
页数:7
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