Control of Lineage-Specific Gene Expression by Functionalized gRNA Barcodes

被引：28
|
作者：
Al'Khafaji, Aziz M. [1 ,2 ]
Deatherage, Daniel [2 ]
Brock, Amy [1 ,2 ]
机构：
[1] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA
[2] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA
来源：
ACS SYNTHETIC BIOLOGY | 2018年 / 7卷 / 10期
基金：
美国国家卫生研究院;
关键词：
lineage tracing; heterogeneity; barcode; clonal dynamics; population variation; CELL HETEROGENEITY; SINGLE CELLS; CANCER; EVOLUTION; DYNAMICS; IDENTIFICATION; TRACKING; MUTATION;
D O I：
10.1021/acssynbio.8b00105
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Lineage tracking delivers essential quantitative insight into dynamic, probabilistic cellular processes, such as somatic tumor evolution and differentiation. Methods for high diversity lineage quantitation rely on sequencing a population of DNA barcodes. However, manipulation of specific individual lineages is not possible with this approach. To address this challenge, we developed a functionalized lineage tracing tool, Control of Lineages by Barcode Enabled Recombinant Transcription (COLBERT), that enables high diversity lineage tracing and lineage-specific manipulation of gene expression. This modular platform utilizes expressed barcode gRNAs to both track cell lineages and direct lineage-specific gene expression.
引用
收藏
页码：2468 / 2474
页数：13
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