Control of Lineage-Specific Gene Expression by Functionalized gRNA Barcodes

被引：28
|
作者：
Al'Khafaji, Aziz M. [1 ,2 ]
Deatherage, Daniel [2 ]
Brock, Amy [1 ,2 ]
机构：
[1] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA
[2] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA
来源：
ACS SYNTHETIC BIOLOGY | 2018年 / 7卷 / 10期
基金：
美国国家卫生研究院;
关键词：
lineage tracing; heterogeneity; barcode; clonal dynamics; population variation; CELL HETEROGENEITY; SINGLE CELLS; CANCER; EVOLUTION; DYNAMICS; IDENTIFICATION; TRACKING; MUTATION;
D O I：
10.1021/acssynbio.8b00105
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Lineage tracking delivers essential quantitative insight into dynamic, probabilistic cellular processes, such as somatic tumor evolution and differentiation. Methods for high diversity lineage quantitation rely on sequencing a population of DNA barcodes. However, manipulation of specific individual lineages is not possible with this approach. To address this challenge, we developed a functionalized lineage tracing tool, Control of Lineages by Barcode Enabled Recombinant Transcription (COLBERT), that enables high diversity lineage tracing and lineage-specific manipulation of gene expression. This modular platform utilizes expressed barcode gRNAs to both track cell lineages and direct lineage-specific gene expression.
引用
收藏
页码：2468 / 2474
页数：13
相关论文
共 50 条
  • [31] Inorganic ions activate lineage-specific gene regulatory networks
    Kersey, Anna L.
    Singh, Irtisha
    Gaharwar, Akhilesh K.
    ACTA BIOMATERIALIA, 2024, 183 : 371 - 386
  • [32] LINEAGE-SPECIFIC AND DIFFERENTIATION-STAGE-SPECIFIC GENE-EXPRESSION IN NORMAL AND LEUKEMIC HUMAN MYELOID CELLS
    BIRNIE, GD
    CLARK, P
    BURNS, JH
    WARNOCK, AM
    JOURNAL OF THE ROYAL SOCIETY OF MEDICINE, 1984, 77 (04) : 289 - 294
  • [33] LINEAGE-SPECIFIC GENE REARRANGEMENT-DELETION - A NONCONSERVATIVE MODEL
    HUEBNER, K
    NOWELL, PC
    CROCE, CM
    CANCER RESEARCH, 1989, 49 (15) : 4071 - 4074
  • [34] LINEAGE-SPECIFIC REGULATION OF CELL-CYCLE GENE-EXPRESSION IN DIFFERENTIATING MYELOID CELLS
    BURGER, C
    WICK, N
    MULLER, R
    JOURNAL OF CELL SCIENCE, 1994, 107 : 2047 - 2054
  • [35] Intron Editing of HSPC Enables Lineage-Specific Expression of Therapeutics
    Seclen, Eduardo
    Lawal, Aminah
    Cho, Hana
    Juillerat, Alexandre
    Pulicani, Sylvain
    Duchateau, Philippe
    Valton, Julien
    MOLECULAR THERAPY, 2024, 32 (04) : 363 - 364
  • [36] Correction of Mpl Deficiency by Lentiviral Vectors with Lineage-Specific Expression
    Modlich, Ute
    Heckl, Dirk
    Wicke, Daniel
    Buesche, Guntram
    Brugman, Martijn
    Meyer, Johann
    Schambach, Axel
    Ballmaier, Matthias
    Baum, Christopher
    HUMAN GENE THERAPY, 2010, 21 (09) : 1183 - 1183
  • [37] Lineage-specific splicing regulation of MAPT gene in the primate brain
    Recinos, Yocelyn
    Bao, Suying
    Wang, Xiaojian
    Phillips, Brittany L.
    Yeh, Yow-Tyng
    Weyn-Vanhentenryck, Sebastien M.
    Swanson, Maurice S.
    Zhang, Chaolin
    CELL GENOMICS, 2024, 4 (06):
  • [38] Expression of lineage-specific developmental genes in pediatric CNS tumors
    Khechoyan, DY
    Rostomily, RC
    Elias, MC
    JOURNAL OF INVESTIGATIVE MEDICINE, 2003, 51 : S99 - S100
  • [39] Correction of Mpl deficiency by lentiviral vectors with lineage-specific expression
    Heckl, Dirk
    Meyer, Johann
    Wicke, Daniel
    Schambach, Axel
    Ballmaier, Matthias
    Baum, Christopher
    Modlich, Ute
    HUMAN GENE THERAPY, 2009, 20 (11) : 1463 - 1463
  • [40] Targeting expression to megakaryocytes and platelets by lineage-specific lentiviral vectors
    Latorre-Rey, L. J.
    Wintterle, S.
    Duetting, S. .
    Kohlscheen, S.
    Abel, T.
    Schenk, F.
    Wingert, S.
    Rieger, M. A.
    Nieswandt, B.
    Heinz, N.
    Modlich, U.
    JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2017, 15 (02) : 341 - 355
12345