Structural Elucidation of in vivo and in vitro Metabolites of Epiberberine in Rat and Its Liver and Intestines by Liquid Chromatography-Tandem Mass Spectrometry

被引：9
|
作者：
Chen, Huai-Xia [1 ]
Huang, Jian-Lin
Li, Juan
机构：
[1] Hubei Univ, Coll Chem & Chem Engn, Wuhan 430062, Peoples R China
来源：
ANALYTICAL LETTERS | 2010年 / 43卷 / 16期
关键词：
Epiberberine; LC-MS; MS; Metabolite; Structural elucidation; HUMAN PLASMA; COPTIDIS-RHIZOMA; BERBERINE; IDENTIFICATION; URINE; ALKALOIDS; MECHANISM; SYSTEM;
D O I：
10.1080/00032711003725607
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The in vivo and in vitro metabolism of epiberberine was investigated using a highly specific and sensitive liquid chromatography-mass spectrometry (LC-MS/MS) method. In vivo samples including rat urine, feces, and plasma samples were collected individually after ingestion of 35mg/kg epiberberine to healthy rats. In vitro samples were prepared by incubating epiberberine with homogenized liver and intestinal flora of rats, respectively. As a result, at least 17, 3 and 5 metabolites were found in rat urine, feces, and plasma, respectively. Additionally, 1 and 3 metabolites were found in the rat intestinal flora and homogenized liver incubation mixtures, respectively.
引用
收藏
页码：2505 / 2517
页数：13
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