Liquid chromatography-tandem mass spectrometry analysis for azaspiracids and their metabolites

被引：0

作者：

Wu Haiyan ^{[1
,2
,3
]}

Guo Mengmeng ^{[1
,2
,3
]}

Zhao Chunxia ^{[1
,2
,3
]}

Tan Zhijun ^{[1
,2
,3
]}

Gu Haifeng ^{[4
]}

Zhai Yuxiu ^{[1
,2
,3
]}

Lu Lina ^{[1
,2
,3
]}

机构：

[1] Minist Agr, Key Lab Testing & Evaluat Aquat Prod Safety & Qua, Qingdao 266071, Peoples R China

[2] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

[3] Minist Agr, Lab Qual & Safety Risk Assessment Aquat Prod Qing, Qingdao 266071, Peoples R China

[4] State Ocean Adm, Inst Oceanog 3, Xiamen 361000, Peoples R China

来源：

CHINESE JOURNAL OF CHROMATOGRAPHY | 2016年 / 34卷 / 04期

基金：

中国国家自然科学基金;

关键词：

liquid chromatography-tandem mass spectrometry (LC-MS/MS); azaspiracids (AZAs); metabolites; qualitative analysis;

D O I：

10.3724/SP.J.1123.2015.11043

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A liquid chromatography-tandem mass spectrometry method was developed for the analysis of azaspiracids and their metabolites. Both target metabolites and non-objective compounds detection methods were investigated, and the secondary mass spectra were compared with the standard spectra libraries to achieve high precision qualitative analysis. As a result, 11 metabolites including AZA-2, 3, 6, 11, 12, 16, 17, 28 and 36, as well as the glutathione conjugated metabolites of AZAs were analyzed in toxin-producing algae, accumulated samples of metabolic experiments and positive samples. Conventional metabolites were unbiased detected by target metabolites detection method, and showed a better response to low concentration metabolites. The method has excellent reproducibility, and is easy for operators. It can be used for the routine monitoring of AZAs.

引用

页码：401 / 406

页数：6

共 17 条

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