Crystallization and preliminary X-ray diffraction analysis of FKBP12 complexed with a new neurotrophic ligand

被引：1
|
作者：
Li, PY
Wang, LW
Wu, BL
Shu, CL
Nie, AH
Shen, BF
Li, S [1 ]
Rao, ZH
机构：
[1] Tsing Hua Univ, Struct Biol Lab, Dept Biol Sci & Technol, Beijing 100084, Peoples R China
[2] Tsing Hua Univ, MOE Lab Prot Sci, Beijing 100084, Peoples R China
[3] Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
[4] Beijing Inst Basic Med Sci, Beijing 100850, Peoples R China
来源：
PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL | 2003年 / 13卷 / 10期
关键词：
FKBP12; (3R)-4-(p-toluenesulfonyl)-1; 4-thiazane-3-carboxylic aeid-L-phenylalanine ethyl ester; complex; X-ray crystallization; ligand; neurotrophic;
D O I：
10.1080/10020070312331344390
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
A novel neurotrophic ligand, (3R)-4-(p-toluenesulfonyl)-1, 4-thiazane-3-carboxylic acid-L-phenylalanine ethyl ester, has been complexed with the FK506 binding protein of 12 kD (FKBP12) and crystallized using the hanging-drop vapor-diffusion method in 0.1 mol/L Hepes Na (PH6.5) solution containing 15% similar to27% PEG10000. Crystals belong to the P2(1) space group, with unit cell parameters of a = 42.0, b = 30.4, c = 42.4 Angstrom, beta = 110.7degrees. The crystals diffract to a 1.8 Angstrom resolution limit.
引用
收藏
页码：765 / 767
页数：3
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