Crystallization and preliminary X-ray diffraction analysis of FKBP12 complexed with a new neurotrophic ligand

被引：1
|
作者：
Li, PY
Wang, LW
Wu, BL
Shu, CL
Nie, AH
Shen, BF
Li, S [1 ]
Rao, ZH
机构：
[1] Tsing Hua Univ, Struct Biol Lab, Dept Biol Sci & Technol, Beijing 100084, Peoples R China
[2] Tsing Hua Univ, MOE Lab Prot Sci, Beijing 100084, Peoples R China
[3] Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
[4] Beijing Inst Basic Med Sci, Beijing 100850, Peoples R China
来源：
PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL | 2003年 / 13卷 / 10期
关键词：
FKBP12; (3R)-4-(p-toluenesulfonyl)-1; 4-thiazane-3-carboxylic aeid-L-phenylalanine ethyl ester; complex; X-ray crystallization; ligand; neurotrophic;
D O I：
10.1080/10020070312331344390
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
A novel neurotrophic ligand, (3R)-4-(p-toluenesulfonyl)-1, 4-thiazane-3-carboxylic acid-L-phenylalanine ethyl ester, has been complexed with the FK506 binding protein of 12 kD (FKBP12) and crystallized using the hanging-drop vapor-diffusion method in 0.1 mol/L Hepes Na (PH6.5) solution containing 15% similar to27% PEG10000. Crystals belong to the P2(1) space group, with unit cell parameters of a = 42.0, b = 30.4, c = 42.4 Angstrom, beta = 110.7degrees. The crystals diffract to a 1.8 Angstrom resolution limit.
引用
收藏
页码：765 / 767
页数：3
相关论文
共 50 条
  • [31] Crystallization and preliminary X-ray diffraction analysis of the extracellular domain of the cell surface antigen CD38 complexed with ganglioside
    Kukimoto, M
    Nureki, O
    Shirouzu, M
    Katada, T
    Hirabayashi, Y
    Sugiya, H
    Furuyama, S
    Yokoyama, S
    Hara-Yokoyama, M
    JOURNAL OF BIOCHEMISTRY, 2000, 127 (02): : 181 - 184
  • [32] Crystallization and preliminary X-ray crystallographic analysis of human phosphodiesterase 12
    Kohno, Tetsuya
    Yamaguchi, Hiroto
    Hakoshima, Toshio
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 520 - 522
  • [33] Purification, crystallization and preliminary X-ray diffraction analysis of thermostable nitrile hydratase
    Tsekoa, TL
    Sayed, MF
    Cameron, RA
    Sewell, BT
    Cowan, DA
    SOUTH AFRICAN JOURNAL OF SCIENCE, 2004, 100 (9-10) : 488 - 490
  • [34] Crystallization and preliminary X-ray diffraction analysis of the hyperthermophilic Sulfolobus islandicus lactonase
    Gotthard, Guillaume
    Hiblot, Julien
    Elias, Mikael
    Chabriere, Eric
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 354 - 357
  • [35] Crystallization and preliminary X-ray diffraction analysis of the hyperthermophilic Sulfolobus solfataricus phosphotriesterase
    Elias, Mikael
    Dupuy, Jerome
    Merone, Luigia
    Lecomte, Claude
    Rossi, Mose
    Masson, Patrick
    Manco, Giuseppe
    Chabriere, Eric
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2007, 63 : 553 - 555
  • [36] Crystallization and preliminary X-ray diffraction analysis of monkey dimeric dihydrodiol dehydrogenase
    El-Kabbani, O
    Chung, RPT
    Ramsland, PA
    Asada, Y
    Ishikura, S
    Usami, N
    Hara, A
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 163 - 164
  • [37] Crystallization and preliminary X-ray diffraction analysis of Pseudomonas aeruginosa phosphorylcholine phosphatase
    Otero, Lisandro H.
    Beassoni, Paola R.
    Domenech, Carlos E.
    Lisa, Angela T.
    Albert, Armando
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 957 - 960
  • [38] Crystallization and preliminary X-ray diffraction analysis of the fructofuranosidase from Xanthophyllomyces dendrorhous
    Polo, Aitana
    Linde, Dolores
    Estevez, Marta
    Fernandez-Lobato, Maria
    Sanz-Aparicio, Julia
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 1441 - 1444
  • [39] Crystallization and preliminary X-ray diffraction analysis of transaldolase from Thermoplasma acidophilum
    Lehwess-Litzmann, Anja
    Neumann, Piotr
    Golbik, Ralph
    Parthier, Christoph
    Tittmann, Kai
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 584 - 586
  • [40] Crystallization and preliminary X-ray diffraction analysis of rat protein tyrosine phosphatase η
    Matozo, Huita C.
    Nascimento, Alessandro S.
    Santos, Maria A. M.
    Iuliano, Rodolfo
    Fusco, Alfredo
    Polikarpov, Igor
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2006, 62 : 923 - 925
12345