High-performance liquid chromatography of some purine and pyrimidine derivatives on silica in hexane-isopropanol-ethylene glycol mobile phases

An alternative approach for the separation of polar solutes in chromatographic systems consisting of unmodified silica and mobile phases containing solvents with limited mutual solubility has been developed. The investigation of ternary mobile phases consisting of hexane, isopropanol and ethylene glycol has shown that the stationary liquid phase is generated dynamically in the pores of silica, even in the mobile phase not saturated with a polar component. If the phase ratio of the column reaches 0.1 partition dominates over adsorption and such mixed partition-adsorption systems show a good column efficiency and peak symmetry of some purine and pyrimidine derivatives. (C) 1999 Elsevier Science B.V. All rights reserved.