AUTOMATED SCREENING SYSTEM FOR PURINE AND PYRIMIDINE METABOLISM DISORDERS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：50
|
作者：
SUMI, S
KIDOUCHI, K
OHBA, S
WADA, Y
机构：
[1] NAGOYA CITY HIGASHI GEN HOSP,DEPT PEDIAT,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN
[2] NAGOYA CITY UNIV,SCH MED,DEPT PEDIAT,MIZUHO KU,NAGOYA,AICHI 467,JAPAN
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 672卷 / 02期
关键词：
D O I：
10.1016/0378-4347(95)00228-B
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An automated screening system for purine and pyrimidine metabolism disorders using high-performance liquid chromatography (HPLC) with column switching is described. The system consists of a reversed-phase column, a cation-exchange column, a column switch, four sets of ultraviolet absorbance detectors, a microcomputer and other conventional equipment. As this system permits the simultaneous determination of urinary erotic acid, uracil, dihydrouracil, pseudouridine, xanthine, 2,8-dihydroxyadenine and succinyladenosine, it offers a useful method for the detection of erotic aciduria, dihydropyrimidine dehydrogenase deficiency, diphydropyrimidinuria, xanthinuria, adenine phosphoribosyltransferase deficiency and adenylosuccinase deficiency.
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页码：233 / 239
页数：7
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