Characterisation of genes differentially expressed in macrophages by virulent and attenuated Mycobacterium tuberculosis through RNA-Seq analysis

被引：18
|
作者：
Lee, Junghwan [1 ,2 ]
Lee, Sung-Gwon [5 ]
Kim, Kee K. [4 ]
Lim, Yun-Ji [1 ,2 ,3 ]
Choi, Ji-Ae [1 ,2 ,3 ]
Cho, Soo-Na [1 ,2 ]
Park, Chungoo [5 ]
Song, Chang-Hwa [1 ,2 ,3 ]
机构：
[1] Chungnam Natl Univ, Coll Med, Dept Microbiol, Daejeon 35015, South Korea
[2] Chungnam Natl Univ, Coll Med, Dept Med Sci, Daejeon 35015, South Korea
[3] Chungnam Natl Univ, Coll Med, Res Inst Med Sci, Daejeon 35015, South Korea
[4] Chungnam Natl Univ, Coll Nat Sci, Dept Biochem, Daejeon 34134, South Korea
[5] Chonnam Natl Univ, Sch Biol Sci & Technol, Gwangju 61186, South Korea
来源：
SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期
基金：
新加坡国家研究基金会;
关键词：
NITRIC-OXIDE SYNTHASE; AMINO-ACID TRANSPORTER-2; ARGININE TRANSPORT; INNATE IMMUNITY; GROWTH; INDUCTION; MECHANISM; IDENTIFICATION; TRANSCRIPTOME; POLARIZATION;
D O I：
10.1038/s41598-019-40814-0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Tuberculosis (TB) remains a global healthcare issue. Understanding the host-pathogen interactions in TB is vital to develop strategies and therapeutic tools for the control of Mycobacterium tuberculosis (Mtb). In this study, transcriptome analyses of macrophages infected with either the virulent Mtb strain H37Rv (Rv) or the avirulent Mtb strain H37Ra (Ra) were carried out and 750 differentially expressed genes (DEGs) were identified. As expected, the DEGs were mainly involved in the induction of innate immune responses against mycobacterial infections. Among the DEGs, solute carrier family 7 member 2 (Slc7 alpha 2) was more strongly expressed in Ra-infected macrophages. Induction of SLC7A2 was important for macrophages to control the intracellular survival of Mtb. Our results imply that SLC7A2 plays an important role in macrophages during Mtb infection. Our findings could prove useful for the development of new therapeutic strategies to control TB infection.
引用
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页数：9
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