Multi-Isotope Capabilities of a Small-Animal Multi-Pinhole SPECT System

The quantitative accuracy and image quality of multi-isotope SPECT is affected by various hardware-related perturbations. The present study evaluates the simultaneous acquisition of multiple isotopes using a multiplexed multi-pinhole SPECT system, assesses the extent of different error sources, and proposes experimental procedures for its objective characterization. Methods: Phantom measurements with single-, dual- and triple-isotope combinations of (99)mTc, In-111, I-123, Lu-177, and Tl-201 were performed with the NanoSPECT/CTPI-us to evaluate system energy resolution, count rate performance, sensitivity, collimator penetration, hardware versus object scatter, spectral crosstalk, spatial resolution, spatial registration accuracy, image uniformity, image noise, and image quality. Results: The intrinsic detector properties were suitable for the simultaneous acquisition of up to 3 isotopes with limitations for count rates exceeding 104 kcps and y-energies lower than 75 keV. Spectral crosstalk between isotopes was more likely mediated by hardware than by source scatter and was strongly dependent on the isotope combination. Simultaneous multi-isotope acquisitions slightly degraded spatial resolution and image uniformity for spatially superimposed but not for spatially separated activity distributions while the background noise level was increased for all multi-isotope studies. For particular isotopes, collimator penetration and x-ray fluorescence contributed a significant portion of error. Conclusion: The NanoSPECT/CTPLus enables the simultaneous acquisition of 3 radioisotopes with high quantitative accuracy and only little loss of image quality when the activity ratio is adapted to isotope-specific count rate sensitivities and when the system calibration is performed with phantoms of appropriate size.