miRMut: Annotation of mutations in miRNA genes from human whole-exome or whole-genome sequencing

Here, we present the miRMut protocol to annotate mutations found in miRNA genes based on whole-exome sequencing (WES) or whole-genome sequencing (WGS) results. The pipeline assigns mutation characteristics, including miRNA gene IDs (miRBase and MirGeneDB), mutation localization within the miRNA pre-cursor structure, potential RNA-binding motif disruption, the ascription of muta-tion according to Human Genome Variation Society (HGVS) nomenclature, and miRNA gene characteristics, such as miRNA gene confidence and miRNA arm bal-ance. The pipeline includes creating tabular and graphical summaries.For complete details on the use and execution of this protocol, please refer to Urbanek-Trzeciak et al. (2020).