Time-gated imaging for multifocus second-harmonic generation microscopy

被引:4
|
作者
Kobayashi, M
Fujita, K
Nakamura, O
Kawata, S
机构
[1] Osaka Univ, Dept Appl Phys, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan
[3] Osaka Univ, Dept Appl Phys, Suita, Osaka 5650871, Japan
[4] RIKEN, Nanophoton Lab, Wako, Saitama 3510198, Japan
来源
REVIEW OF SCIENTIFIC INSTRUMENTS | 2005年 / 76卷 / 07期
关键词
D O I
10.1063/1.1947047
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
We developed a scanning second-harmonic generation (SHG) microscope with a time-gated image intensifier. The combination of a microlens array scanner and the gated image intensifier allows us to perform simultaneous time-resolved detection of SHG at multiple positions in a sample. By tuning the time delay of the gated detection from pulsed laser illumination by a mode-locked Ti:sapphire laser, background noise originating from the image intensifier dark current, fluorescence from the sample, and stray light are effectively reduced. (c) 2005 American Institute of Physics.
引用
收藏
页数:4
相关论文
共 50 条
  • [41] Fabrication of Ion-Shaped Anisotropic Nanoparticles and their Orientational Imaging by Second-Harmonic Generation Microscopy
    Abdallah Slablab
    Tero J. Isotalo
    Jouni Mäkitalo
    Léo Turquet
    Pierre-Eugène Coulon
    Tapio Niemi
    Christian Ulysse
    Mathieu Kociak
    Dominique Mailly
    Giancarlo Rizza
    Martti Kauranen
    Scientific Reports, 6
  • [42] Fabrication of Ion-Shaped Anisotropic Nanoparticles and their Orientational Imaging by Second-Harmonic Generation Microscopy
    Slablab, Abdallah
    Isotalo, Tero J.
    Makitalo, Jouni
    Turquet, Leo
    Coulon, Pierre-Eugene
    Niemi, Tapio
    Ulysse, Christian
    Kociak, Mathieu
    Mailly, Dominique
    Rizza, Giancarlo
    Kauranen, Martti
    SCIENTIFIC REPORTS, 2016, 6
  • [43] SECOND-HARMONIC GENERATION IN MOLYBDATES
    JEGGO, CR
    JOURNAL OF PHYSICS PART C SOLID STATE PHYSICS, 1972, 5 (11): : L133 - +
  • [44] Quasipatterns in second-harmonic generation
    Longhi, S
    PHYSICAL REVIEW E, 1999, 59 (01) : R24 - R27
  • [45] Adiabatic second-harmonic generation
    Leshem, Anat
    Meshulam, Guilia
    Porat, Gil
    Arie, Ady
    OPTICS LETTERS, 2016, 41 (06) : 1229 - 1232
  • [46] Quasipatterns in second-harmonic generation
    Phys Rev E., 1-B (R24):
  • [47] Spectral imaging of breast fibroadenoma using second-harmonic generation
    Zheng, Liqin
    Wang, Yuhua
    TWELFTH INTERNATIONAL CONFERENCE ON PHOTONICS AND IMAGING IN BIOLOGY AND MEDICINE (PIBM 2014), 2014, 9230
  • [48] Measurement of muscle disease by quantitative second-harmonic generation imaging
    Plotnikov, Sergey V.
    Kenny, Anne M.
    Walsh, Stephen J.
    Zubrowski, Beata
    Joseph, Cherian
    Scranton, Victoria L.
    Kuchel, George A.
    Dauser, Deborah
    Xu, Manshan
    Pilbeam, Carol C.
    Adams, Douglas J.
    Dougherty, Robert P.
    Campagnola, Paul J.
    Mohler, William A.
    JOURNAL OF BIOMEDICAL OPTICS, 2008, 13 (04)
  • [49] Chiral imaging of collagen by second-harmonic generation circular dichroism
    Lee, H.
    Huttunen, M. J.
    Hsu, K. -J.
    Partanen, M.
    Zhuo, G. -Y.
    Kauranen, M.
    Chu, S. -W.
    BIOMEDICAL OPTICS EXPRESS, 2013, 4 (06): : 909 - 916
  • [50] Second-Harmonic Generation Imaging of Membrane Potential with Retinal Analogues
    Theer, Patrick
    Denk, Winfried
    Sheves, Mordechai
    Lewis, Aaron
    Detwiler, Peter B.
    BIOPHYSICAL JOURNAL, 2011, 100 (01) : 232 - 242