Time-gated imaging for multifocus second-harmonic generation microscopy

被引:4
|
作者
Kobayashi, M
Fujita, K
Nakamura, O
Kawata, S
机构
[1] Osaka Univ, Dept Appl Phys, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan
[3] Osaka Univ, Dept Appl Phys, Suita, Osaka 5650871, Japan
[4] RIKEN, Nanophoton Lab, Wako, Saitama 3510198, Japan
来源
REVIEW OF SCIENTIFIC INSTRUMENTS | 2005年 / 76卷 / 07期
关键词
D O I
10.1063/1.1947047
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
We developed a scanning second-harmonic generation (SHG) microscope with a time-gated image intensifier. The combination of a microlens array scanner and the gated image intensifier allows us to perform simultaneous time-resolved detection of SHG at multiple positions in a sample. By tuning the time delay of the gated detection from pulsed laser illumination by a mode-locked Ti:sapphire laser, background noise originating from the image intensifier dark current, fluorescence from the sample, and stray light are effectively reduced. (c) 2005 American Institute of Physics.
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页数:4
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