Regeneration of peach (Prunus persica L. Batsch) cultivars and Prunus persica x Prunus dulcis rootstocks via organogenesis

被引:31
|
作者
Perez-Jimenez, Margarita [1 ]
Carrillo-Navarro, Antonio [1 ]
Cos-Terrer, Jose [1 ]
机构
[1] Inst Murciano Invest & Desarrollo Agr & Alimentar, Dept Hort, Murcia 30150, Spain
关键词
Morphogenesis; Prunus; 6-Benzyladenine; Indolebutyric acid; alpha-Naphthalene acetic; Somatic shoots; FLUORESCENT PROTEIN GFP; PLANT-REGENERATION; EMBRYOS; TRANSFORMATION; GROWTH; LEAVES;
D O I
10.1007/s11240-011-0011-y
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Somatic peach plants were regenerated from callus derived from the base of stem explants of the scion cultivars 'UFO-3', 'Maruja', 'Flariba' and 'Alice Bigi', and the peach 9 almond rootstocks 'Garnem' and 'GF677'. A protocol for organogenic plant regeneration was developed using three culture media containing different concentrations of 6-benzyladenine (BA) and indolebutyric acid to produce organogenic calli. Shoots were obtained from sliced calli after their transfer to a differentiation culture medium containing 2 mg l(-1) BA and 1 mg l(-1) alpha-naphthalene acetic acid. Using this procedure, up to 29 regenerated plants per callus were obtained. The highest regeneration rate was obtained with the peach x almond rootstocks. This work provides an effective protocol that could be utilized for peach transformation research.
引用
收藏
页码:55 / 62
页数:8
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