Regeneration of peach (Prunus persica L. Batsch) cultivars and Prunus persica x Prunus dulcis rootstocks via organogenesis

被引：31
|
作者：
Perez-Jimenez, Margarita [1 ]
Carrillo-Navarro, Antonio [1 ]
Cos-Terrer, Jose [1 ]
机构：
[1] Inst Murciano Invest & Desarrollo Agr & Alimentar, Dept Hort, Murcia 30150, Spain
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2012年 / 108卷 / 01期
关键词：
Morphogenesis; Prunus; 6-Benzyladenine; Indolebutyric acid; alpha-Naphthalene acetic; Somatic shoots; FLUORESCENT PROTEIN GFP; PLANT-REGENERATION; EMBRYOS; TRANSFORMATION; GROWTH; LEAVES;
D O I：
10.1007/s11240-011-0011-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Somatic peach plants were regenerated from callus derived from the base of stem explants of the scion cultivars 'UFO-3', 'Maruja', 'Flariba' and 'Alice Bigi', and the peach 9 almond rootstocks 'Garnem' and 'GF677'. A protocol for organogenic plant regeneration was developed using three culture media containing different concentrations of 6-benzyladenine (BA) and indolebutyric acid to produce organogenic calli. Shoots were obtained from sliced calli after their transfer to a differentiation culture medium containing 2 mg l(-1) BA and 1 mg l(-1) alpha-naphthalene acetic acid. Using this procedure, up to 29 regenerated plants per callus were obtained. The highest regeneration rate was obtained with the peach x almond rootstocks. This work provides an effective protocol that could be utilized for peach transformation research.
引用
收藏
页码：55 / 62
页数：8
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