Expression of Multidrug Efflux Pump Genes acrAB-tolC, mdfA, and norE in Escherichia coli Clinical Isolates as a Function of Fluoroquinolone and Multidrug Resistance

被引：158
|
作者：
Swick, Michelle C. [1 ,2 ]
Morgan-Linnell, Sonia K. [1 ]
Carlson, Kimberly M. [1 ,3 ]
Zechiedrich, Lynn [1 ,2 ,3 ]
机构：
[1] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
[2] Baylor Coll Med, Interdept Program Cell & Mol Biol, Houston, TX 77030 USA
[3] Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2011年 / 55卷 / 02期
关键词：
16S RIBOSOMAL-RNA; HYDROPHILIC FLUOROQUINOLONES; QEPA; AMINOGLYCOSIDES; METHYLATION; BACTERIA;
D O I：
10.1128/AAC.00996-10
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In a single quantitative study, we measured acrA, acrB, tolC, mdfA, and norE expression in Escherichia coli clinical isolates by using real-time PCR. acrA and acrB overexpression strongly correlated with fluoroquinolone and multidrug resistance; tolC, mdfA, and norE expression did not. The order of abundance of efflux pump transcripts in all fluoroquinolone-susceptible isolates was tolC (highest), then acrA and acrB, and then mdfA and norE. Our findings suggest acrAB overexpression is an indicator of multidrug resistance.
引用
收藏
页码：921 / 924
页数：4
相关论文
共 50 条
  • [31] The C-Terminal Domain of AcrA Is Essential for the Assembly and Function of the Multidrug Efflux Pump AcrAB-TolC
    Ge, Qiang
    Yamada, Yoichi
    Zgurskaya, Helen
    JOURNAL OF BACTERIOLOGY, 2009, 191 (13) : 4365 - 4371
  • [32] The Role of AcrAB-TolC Efflux Pump in Mediating Fluoroquinolone Resistance in Naturally Occurring Salmonella Isolates from China
    Shen, Jinling
    Yang, Baowei
    Gu, Qiang
    Zhang, Guodong
    Yang, Jielin
    Xue, Feng
    Shao, Jingdong
    Yi, Xiaojuan
    Jiang, Yuan
    FOODBORNE PATHOGENS AND DISEASE, 2017, 14 (12) : 728 - 734
  • [33] Resveratrol controls Escherichia coli growth by inhibiting the AcrAB-TolC efflux pump
    Hwang, Dahyun
    Lim, Young-Hee
    FEMS MICROBIOLOGY LETTERS, 2019, 366 (04)
  • [34] Total Alkaloids of Sophorea alopecuroides-induced Down-regulation of AcrAB-ToLC Efflux Pump Reverses Susceptibility to Ciprofloxacin in Clinical Multidrug Resistant Escherichia coli isolates
    Zhou, Xuezhang
    Jia, Fang
    Liu, Xiaoming
    Wang, Yujiong
    PHYTOTHERAPY RESEARCH, 2012, 26 (11) : 1637 - 1643
  • [35] Effect of Transcriptional Activators SoxS, RobA, and RamA on Expression of Multidrug Efflux Pump AcrAB-TolC in Enterobacter cloacae
    Perez, Astrid
    Poza, Margarita
    Aranda, Jesus
    Latasa, Cristina
    Javier Medrano, Francisco
    Tomas, Mara
    Romero, Antonio
    Lasa, Inigo
    Bou, German
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2012, 56 (12) : 6256 - 6266
  • [36] Atomistic and Coarse-Grain Computer Simulations of the Assembled AcrAB-TolC Multidrug Efflux Pump
    Schmidt, Thomas H.
    Fischer, Nadine
    Raunest, Martin
    Kandt, Christian
    BIOPHYSICAL JOURNAL, 2011, 100 (03) : 146 - 146
  • [37] Optimization of pyridylpiperazine-based inhibitors of the Escherichia coli AcrAB-TolC efflux pump
    Compagne, Nina
    Jimenez-Castellanos, Juan-Carlos
    Meurillon, Virginie
    Pradel, Elizabeth
    Da Cruz, Anais Vieira
    Piveteau, Catherine
    Biela, Alexandre
    Eveque, Maxime
    Leroux, Florence
    Deprez, Benoit
    Willand, Nicolas
    Hartkoorn, Ruben C.
    Flipo, Marion
    EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2023, 259
  • [38] AcrS/EnvR represses expression of the acrAB multidrug efflux genes in Escherichia coli
    Hirakawa, Hidetada
    Takumi-Kobayashi, Asuka
    Theisen, Ulrike
    Hirata, Takahiro
    Nishino, Kunihiko
    Yamaguchi, Akihito
    JOURNAL OF BACTERIOLOGY, 2008, 190 (18) : 6276 - 6279
  • [39] Biased partitioning of the multidrug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity
    Bergmiller, Tobias
    Andersson, Anna M. C.
    Tomasek, Kathrin
    Balleza, Enrique
    Kiviet, Daniel J.
    Hauschild, Robert
    Tkacik, Gasper
    Guet, Calin C.
    SCIENCE, 2017, 356 (6335) : 309 - 311
  • [40] Genetic assessment of the role of AcrB βhairpins in the assembly of the TolC-AcrAB multidrug efflux pump of Escherichia coli
    Weeks, Jon W.
    Bavro, Vassiliy N.
    Misra, Rajeev
    MOLECULAR MICROBIOLOGY, 2014, 91 (05) : 965 - 975
12345