Expression of Multidrug Efflux Pump Genes acrAB-tolC, mdfA, and norE in Escherichia coli Clinical Isolates as a Function of Fluoroquinolone and Multidrug Resistance

被引：158
|
作者：
Swick, Michelle C. [1 ,2 ]
Morgan-Linnell, Sonia K. [1 ]
Carlson, Kimberly M. [1 ,3 ]
Zechiedrich, Lynn [1 ,2 ,3 ]
机构：
[1] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
[2] Baylor Coll Med, Interdept Program Cell & Mol Biol, Houston, TX 77030 USA
[3] Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2011年 / 55卷 / 02期
关键词：
16S RIBOSOMAL-RNA; HYDROPHILIC FLUOROQUINOLONES; QEPA; AMINOGLYCOSIDES; METHYLATION; BACTERIA;
D O I：
10.1128/AAC.00996-10
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In a single quantitative study, we measured acrA, acrB, tolC, mdfA, and norE expression in Escherichia coli clinical isolates by using real-time PCR. acrA and acrB overexpression strongly correlated with fluoroquinolone and multidrug resistance; tolC, mdfA, and norE expression did not. The order of abundance of efflux pump transcripts in all fluoroquinolone-susceptible isolates was tolC (highest), then acrA and acrB, and then mdfA and norE. Our findings suggest acrAB overexpression is an indicator of multidrug resistance.
引用
收藏
页码：921 / 924
页数：4
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