Transfection of mammalian cells by electroporation

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作者：
不详
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来源：
NATURE METHODS | 2006年 / 3卷 / 01期
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中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Pulsed electrical fields can be used to introduce DNA into a wide variety of animal cells(1,2). Electroporation works well with cell Lines that are refractive to other techniques, such as calcium phosphate-DNA coprecipitation. But as with other transfection methods, the optimal conditions for electroporation of untested cell tines must be determined experimentally.
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页码：67 / 68
页数：2
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