Transfection of mammalian cells by electroporation

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Q5 [生物化学];
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071010 ; 081704 ;
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Pulsed electrical fields can be used to introduce DNA into a wide variety of animal cells(1,2). Electroporation works well with cell Lines that are refractive to other techniques, such as calcium phosphate-DNA coprecipitation. But as with other transfection methods, the optimal conditions for electroporation of untested cell tines must be determined experimentally.
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页码:67 / 68
页数:2
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