Impact of DNA polymerases and their buffer systems on quantitative real-time PCR

被引：45
|
作者：
Wolffs, P [1 ]
Grage, H [1 ]
Hagberg, O [1 ]
Rådström, P [1 ]
机构：
[1] Lund Univ, Lund Inst Technol, SE-22100 Lund, Sweden
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 01期
关键词：
D O I：
10.1128/JCM.42.1.408-411.2004
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.
引用
收藏
页码：408 / 411
页数：4
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