Impact of DNA polymerases and their buffer systems on quantitative real-time PCR

被引：45

作者：

Wolffs, P ^{[1
]}

Grage, H ^{[1
]}

Hagberg, O ^{[1
]}

Rådström, P ^{[1
]}

机构：

[1] Lund Univ, Lund Inst Technol, SE-22100 Lund, Sweden

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 01期

关键词：

D O I：

10.1128/JCM.42.1.408-411.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.

引用

页码：408 / 411

页数：4

共 50 条

[21] Genomic DNA functions as a universal external standard in quantitative real-time PCR
Yun, James J.
Heisler, Lawrence E.
Hwang, Irene I. L.
Wilkins, Olivia
Lau, Suzanne K.
Hyrcza, Martin
Jayabalasingham, Bamini
Jin, Jing
McLaurin, JoAnne
Tsao, Ming-Sound
Der, Sandy D.
NUCLEIC ACIDS RESEARCH, 2006, 34 (12) : e85
[22] A real-time PCR method for the quantitative detection of HBV viral DNA.
Rhoads, JA
Riesing, KS
Pabich, EK
Robinson, JM
Mullen, CR
JOURNAL OF MOLECULAR DIAGNOSTICS, 2005, 7 (05): : 669 - 670
[23] Quantitative detection of residual porcine host cell DNA by real-time PCR
Chang, Jen-Ting
Chen, Yu-Chen
Chou, Yu-Chi
Wang, Shih-Rong
BIOLOGICALS, 2014, 42 (02) : 74 - 78
[24] Development of a real-time PCR assay for quantitative detection of Encephalitozoon intestinalis DNA
Menotti, J
Cassinat, B
Sarfati, C
Liguory, O
Derouin, F
Molina, JM
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (04) : 1410 - 1413
[25] Quantification of Neurospora crassa mitochondrial DNA using quantitative real-time PCR
Monteiro, J.
Videira, A.
Pereira, F.
LETTERS IN APPLIED MICROBIOLOGY, 2020, 71 (02) : 171 - 178
[26] Evaluation of uncertainty in quantitative real-time PCR
Love, John L.
Scholes, Paula
Gilpin, Brent
Savill, Marion
Lin, Susan
Samuel, Laly
JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 67 (02) : 349 - 356
[27] Gene dosage by quantitative real-time PCR
Boulay, JL
Reuter, J
Ritschard, R
Terracciano, L
Herrmann, R
Rochlitz, C
BIOTECHNIQUES, 1999, 27 (02) : 228 - +
[28] Quantitative detection of periodontopathogens by real-time PCR
Nonnenmacher, C
Dalpke, A
Mutters, R
Heeg, K
JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 59 (01) : 117 - 125
[29] Real-time quantitative PCR of telomere length
Gil, ME
Coetzer, TL
MOLECULAR BIOTECHNOLOGY, 2004, 27 (02) : 169 - 172
[30] Real-time quantitative PCR for toxoplasmosis diagnosis
Ordinaire, I
Simon, A
Fréalle, E
Soula, F
Valat, AS
Rouland, V
Subtil, D
Dei-Cas, E
Camus, D
Delhaes, L
ANNALES DE BIOLOGIE CLINIQUE, 2005, 63 (01) : 67 - 73

← 1 2 3 4 5 →