Improvement of properties of Pseudomonas sp. lipase in resolution of 2-octanol

被引：0
|
作者：
Wang, Z
Quan, J
Weng, L
Zheng, LY
Liu, N
Dong, H
Cao, SG [1 ]
机构：
[1] Jilin Univ, Key Lab Mol Enzymol & Engn, Educ Minist, Changchun 130023, Peoples R China
[2] Jilin Univ, Coll Life Sci, Changchun 130023, Peoples R China
来源：
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES | 2003年 / 19卷 / 04期
关键词：
lipase; surfactant; salt hydrate pair; esterification; enantioselectivity;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel preparation method was developed to form a surfactant-lipase complex for the resolution of 2-octanol in a solvent-free system. The E value was improved from 6.64 to 120.2. The lipase modified by the anionic surfactant possessed a low solubility in the solvent-free system, which was beneficial to the recovery and the repeated usage of the lipase. The surfactant-lipase complex maintained a high enantioselectivity after five cycles of usage. The effect of water on both the activity and the enantioselectivity of the lipase has also been investigated; the direct addition of a salt hydrate pair of Na4P2O7. H2O and Na4P2O7 can dramatically activate the modified enzyme.
引用
收藏
页码：446 / 449
页数：4
相关论文
共 50 条
  • [41] Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization
    Liu, Ying
    Guo, Chen
    Sun, Xi-Tong
    Liu, Chun-Zhao
    BIORESOURCE TECHNOLOGY, 2013, 142 : 415 - 419
  • [42] Effects of Diesel and 2-Octanol on Water-carrying Properties and Ultrafine Coal Flotation
    Zhu, Jinbo
    Zhu, Hongzheng
    Wang, Haiyan
    Valdivieso, Alejandro Lopez
    Xu, Wenyu
    Song, Qi
    Wang, Hainan
    INTERNATIONAL JOURNAL OF COAL PREPARATION AND UTILIZATION, 2021, 41 (02) : 98 - 107
  • [43] EXCESS PROPERTIES IN BINARY-LIQUID MIXTURES - CHLOROBENZENE + 2-OCTANOL AND TETRAHYDROFURAN + BROMOBENZENE
    AGARWAL, CB
    SHARMA, P
    INDIAN JOURNAL OF PURE & APPLIED PHYSICS, 1990, 28 (04) : 197 - 199
  • [44] USE OF (S)-ALPHA-METHYLBENZYLAMINE IN THE RESOLUTION OF RACEMIC 2-OCTANOL AND ALPHA-METHYLBENZYL ALCOHOL
    REYES, A
    JUARISTI, E
    SYNTHETIC COMMUNICATIONS, 1995, 25 (07) : 1053 - 1058
  • [45] Resolution of (R,S)-2-octanol by Penicillium expansum PED-03 lipase immobilized on modified ultrastable-Y molecular sieve in microaqueous media
    Dai, Da-Zhang
    Xia, Li-Ming
    PROCESS BIOCHEMISTRY, 2006, 41 (06) : 1455 - 1460
  • [46] Optical resolution of RS-(±)-mandelic acid by Pseudomonas sp.
    Kim, BY
    Hwang, KC
    Song, HS
    Chung, N
    Bang, WG
    BIOTECHNOLOGY LETTERS, 2000, 22 (23) : 1871 - 1875
  • [47] Optical resolution of RS-(±)-mandelic acid by Pseudomonas sp.
    Byung-Yong Kim
    Ki-Chul Hwang
    Hee-Sang Song
    Namhyun Chung
    Won-Gi Bang
    Biotechnology Letters, 2000, 22 : 1871 - 1875
  • [48] Studies on the factors that affect stereoselective esterification of (R,S) 2-octanol with octanoic acid catalyzed by lipase in organic solvent
    Yang, H
    Cao, SG
    Han, SP
    Huang, ZL
    Yang, TS
    Xu, JL
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1996, 59 (02) : 177 - 186
  • [49] A novel alkaline, thermostable, protease-free lipase from Pseudomonas sp.
    Neelima Kulkarni
    R.V. Gadre
    Biotechnology Letters, 1999, 21 : 897 - 899
  • [50] A novel alkaline, thermostable, protease-free lipase from Pseudomonas sp.
    Kulkarni, N
    Gadre, RV
    BIOTECHNOLOGY LETTERS, 1999, 21 (10) : 897 - 899
12345